摘要
This work aimed to investigate the psychobiotic effects of six bacterial strains on the mind and behavior of male Wistar rats. The probiotic (PRO) group (n=7) were rats pre-treated with antibiotics for 7 days followed by 14-day probiotic administration, antibiotics (ANT) group (n=7) were rats treated with antibiotics for 21 days without probiotics. The control (CON) group (n=7) were rats that received sham treatment for 21 days. The six bacterial strains with probiotic properties were mostly isolated from Thai fermented foods; Pedicoccus pentosaceus WS11, Lactobacillus plantarum SK321, L. fermentum SK324, L. brevis TRBC 3003, Bifidobacterium adolescentis TBRC 7154 and Lactococcus lactis subsp. lactis TBRC 375. The probiotics were freeze-dried into powder (6×109 CFU/5 g) and administered to the PRO group via oral gavage. Behavioral tests were performed. The PRO group displayed significantly reduced anxiety level and increased locomotor function using a marble burying test and open field test, respectively and significantly improved short-term memory performance using a novel object recognition test. Antibiotics significantly reduced microbial counts in rat feces in the ANT group by 100 fold compared to the PRO group. Probiotics significantly enhanced antioxidant enzymatic and non-enzymatic defenses in rat brains as assessed using catalase activity and ferric reducing antioxidant power assay, respectively. Probiotics also showed neuroprotective effects with less pyknotic cells and lower frequency of vacuolization in cerebral cortex. This multi-strain probiotic formulation from Thai fermented foods may offer a potential to develop psychobiotic-rich functional foods to modulate human mind and behaviors.
摘要译文
这项工作旨在调查六种细菌菌株对雄性Wistar大鼠心理和行为的心理作用。益生菌（PRO）组（n = 7）为接受抗生素预处理的大鼠，共7天，然后进行了14天的益生菌给药；抗生素（ANT）组（n = 7），为接受不含益生菌的抗生素，经过21天的大鼠。对照组（CON）组（n = 7）为接受假手术21天的大鼠。六种具有益生菌特性的细菌菌株大部分是从泰国发酵食品中分离出来的。戊糖双球菌WS11，植物乳杆菌SK321，发酵乳杆菌SK324，短乳杆菌TRBC 3003，青春双歧杆菌TBRC 7154和乳酸乳球菌亚种。乳酸菌TBRC375。将益生菌冷冻干燥，制成粉末（6×109 CFU / 5 g），并通过管饲法给药至PRO组。进行行为测试。 PRO组分别使用大理石掩埋试验和开放视野试验显着降低了焦虑水平并增强了运动功能，并通过新型物体识别试验显着改善了短期记忆性能。与PRO组相比，ANT组中的抗生素可将大鼠粪便中的微生物数量显着降低100倍。分别使用过氧化氢酶活性和铁还原性抗氧化能力测定法评估，益生菌可显着增强大鼠脑中的抗氧化酶和非酶防御能力。益生菌还显示出神经保护作用，即较少的解公济细胞和较低的大脑皮层空泡频率。来自泰国发酵食品的这种多菌株益生菌配方可能为开发富含精神生物的功能食品提供潜力，以调节人类的思想和行为。
Luang-In; Vijitra;Katisart; Teeraporn;Konsue; Ampa;Nudmamud-Thanoi; Sutisa;Narbad; Arjan;Saengha; Worachot;Wangkahart; Eakapol;Pumriw; Supaporn;Samappito; Wannee;Ma; Nyuk Ling;. Psychobiotic Effects of Multi-Strain Probiotics Originated from Thai Fermented Foods in a Rat Model[J]. Korean Journal for Food Science of Animal Resources, 2020,40(6): 1014-1032