摘要
This study was carried out to investigate synthetic extender for semen cryopreservation of Jeju Native Black Bull. The semen was collected using an artificial vagina and transported to the laboratory. The semen was diluted 1:1 by Tris-Egg yolk extender and contrifuged in 1,500 rpm for 15 minutes. The supernatant was removed. The pellect was diluted to final sperm concentration of 2×108/ml'>2×108/ml2×108/ml by doubling in every 30 minutes at 4∘C'>4∘C4∘C 4^{\circ}C cold chamber. The semen was equilibrated for 4 hours at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm for 10 minutes. The height and duration affect the freezing speed by temperature. The frozen straw was plunged to LN2'>LN2LN2 id="MathJax-Element-3" LN_2 . The presented straws were examined the viability and motility after thawed at 37∘C'>37∘C37∘C id="MathJax-Element-4" 37^{\circ}C water bath. Frozen-thawed sperm were evaluated sperm viability, membrane integrity and acrosome integrity. Post-thawed sperm viability has been significantly higher (p<0.05) in fresh sperm (93.27±1.62'>93.27±1.6293.27±1.62 id="MathJax-Element-5" 93.27{\pm}1.62% ) than frozen-thawed sperm (73.34±3.27'>73.34±3.2773.34±3.27 id="MathJax-Element-6" 73.34{\pm}3.27% ). However, there were no significant differences between fresh and frozen-thawed dead cell rate (7.35±2.63'>7.35±2.637.35±2.63 id="MathJax-Element-7" 7.35{\pm}2.63 vs, 13.71±2.85'>13.71±2.8513.71±2.85 id="MathJax-Element-8" 13.71{\pm}2.85 ). In sperm motility, between Triladyl and AndroMed Extender, there was no significant different (72.86±2.83'>72.86±2.8372.86±2.83 id="MathJax-Element-9" 72.86{\pm}2.83 vs, 81.47±2.48'>81.47±2.4881.47±2.48 id="MathJax-Element-10" 81.47{\pm}2.48 ), similarly, the dead cell rates was similar (18.41±3.42'>18.41±3.4218.41±3.42 id="MathJax-Element-11" 18.41{\pm}3.42% and 17.26±4.25'>17.26±4.2517.26±4.25 id="MathJax-Element-12" 17.26{\pm}4.25 ). The results of our study suggest that AndroMed to the freezing extender showed more positive effect on the frozen-thawed spermatozoa in Jeju Native Black bull semen.
摘要译文
这项研究的目的是研究用于济州本地黑牛精液冷冻保存的合成补充剂。使用人造阴道收集精液并运送到实验室。用Tris-Egg蛋黄补充剂将精液以1：1的比例稀释，并在1,500 rpm下稀释15分钟。除去上清液。通过在30°C'> 4°C4°C 4°C的条件下每30分钟加倍，将小动物稀释至最终精子浓度为2×108 / ml'> 2×108 / ml2×108 / ml室。将精液在冷室中平衡4小时，并装入0.5ml吸管中。将精液吸管置于5厘米以上10分钟。高度和持续时间会因温度而影响冷冻速度。将冷冻的秸秆插入到LN2'> LN2LN2 id = MathJax-Element-3 LN_2。在37°C'> 37°C37°C id = MathJax-Element-4 37 ^ {-circ} C水浴中融化后，检查了提出的秸秆的活力和运动性。对冷冻-解冻的精子进行精子活力，膜完整性和顶体完整性评估。融化后精子的活力在新鲜精子中显着更高（p <0.05）（93.27±1.62'> 93.27±1.6293.27±1.62 id = MathJax-Element-5 93.27 {-pm} 1.62％）。 （73.34±3.27'> 73.34±3.2773.34±3.27 id = MathJax-Element-6 73.34 {-pm} 3.27％）。然而，新鲜和冷冻-解冻的死细胞率之间没有显着差异（7.35±2.63'> 7.35±2.637.35±2.63 id = MathJax-Element-7 7.35 {-pm} 2.63 vs. 13.71±2.85'> 13.71 ±2.8513.71±2.85 id = MathJax-Element-8 13.71 {-pm} 2.85）。 Triladyl和AndroMed Extender之间的精子运动力没有显着差异（72.86±2.83'> 72.86±2.8372.86±2.83 id = MathJax-Element–9 72.86 {-pm} 2.83 vs，81.47±2.48'> 81.47± 2.4881.47±2.48 id = MathJax-Element-10 11 81.41 { —pm} 3.42％和17.26±4.25'> 17.26±4.2517.26±4.25 id = MathJax-Element-12 17.26 {-pm} 4.25）。我们的研究结果表明，AndroMed对冷冻补充剂的显示对济州本地黑牛精液中冷冻解冻的精子有更积极的作用。
Cho; Sang-Rae;Cho; In-Chel;Han; Sang-Hyun;Kang; Tae-Young;Cho; Won-Mo;Chae; Hyun-Seok;Kim; Nam-Young;Park; Yong-Sang;Kang; Yong-Jun;Kim; Young-Hoon;Ko; Yeoung-Gyu;Kim; Hyun-Jong;Ko; Moon-Suck;. Comparison of AndroMed and Triladyl Extender for Freezing of Jeju Native Black Bull Semen[J]. Reproductive and Developmental Biology, 2013,37(3): 155-159