摘要
One hundred and twenty 25-day-old barrows with BW of 7.49±0.51 kg were used in a 42-d experiment to evaluate the effects of protease in diets with different levels of soybean meal (SBM) on growth performance, diarrhea incidence, nutrient digestibility, blood metabolites, and digestive enzyme activity. The pigs were allotted to 6 treatment groups following a 2 (two SBM levels) ×3 (three protease levels) factorial arrangement. Two SBM levels included normal SBM (0 to 14 d, 10%; 15 to 42 d, 22%) and low SBM (0 to 14 d, 4%; 15 to 42 d, 16%), and added protease levels included 0, 150 or 300 mg/kg (0, 1,500 and 3,000 U/kg) of diet. Blood, organ and digesta samples were collected at the end of the experiment, and fecal samples were taken from d 38 to 41. The low SBM level diet significant decreased ADFI (P < 0.01), ADG (P < 0.01), and G:F (P < 0.01) throughout the experiment, except the G:F from 29 to 42 d (P > 0.05). Protease in the normal and low SBM diets increased ADG (P < 0.05) and G:F (P < 0.01) from 1 to 14 d Diarrhea rate was significantly reduced by SBM levels and protease (P < 0.05). An SBM level and protease interaction was observed on digestibilities of dry matter (DM), crude protein (CP), crude ash, organ matter (OM) and gross energy (GE) (P < 0.01). The supplementation of protease in normal and low SBM level diets significantly increased nutrients digestibility (P < 0.01), protease increased the digestibilities of majority of amino acids (P < 0.05). Protease in the normal diets reduced serum urea nitrogen (SUN) (P < 0.01). Low SBM diet reduced the liver relative weight (P < 0.05), supplementation of 150 mg/kg protease to the normal SBM diet increased pancreas relative weight (P < 0.05) and protease in both normal and low SBM diets increased intestine relative weight (P < 0.01). Trypsin activity was reduced by low SBM (P < 0.05), while protease enhanced the activity (P < 0.05). An SBM level and protease level interaction was observed on trypsin and chymotrypsin activities (P < 0.01). In conclusion, protease supplementation in low SBM diet could partly complement the digestible AAs to the piglets fed diet with reduced crude protein.
摘要译文
在42天的实验中，使用120个体重在7.49±0.51 kg的25天大的公猪，评估了不同豆粕（SBM）日粮中蛋白酶对生长性能，腹泻发生率，营养素的影响消化率，血液代谢产物和消化酶活性。按照2（两个SBM水平）×3（三个蛋白酶水平）因子分解法将猪分配到6个处理组。两种SBM水平包括正常SBM（0至14 d，10％； 15到42 d，22％）和低SBM（0到14 d，4％）； 15至42 d，16％），添加的蛋白酶水平包括0、150或300 mg / kg（0、1,500和3,000 U / kg）的饮食。在实验结束时采集血液，器官和消化道样品，并在38至41天之间采集粪便样品。低SBM水平饮食显着降低了ADFI（P <0.01），ADG（P <0.01）和G：在整个实验过程中，F（P <0.01），但G：F从29到42 d（P> 0.05）除外。正常和低SBM日粮中的蛋白酶从1到14 d可使ADG（P <0.05）和G：F（P <0.01）增加，SBM和蛋白酶的腹泻率显着降低（P <0.05）。干物质（DM），粗蛋白（CP），粗灰分，器官物质（OM）和总能量（GE）的消化率观察到SBM水平和蛋白酶的相互作用（P <0.01）。正常和低SBM水平日粮中添加蛋白酶可显着提高养分消化率（P <0.01），蛋白酶可提高大多数氨基酸的消化率（P <0.05）。正常饮食中的蛋白酶会降低血清尿素氮（SUN）（P <0.01）。低SBM日粮可降低肝脏相对体重（P <0.05），正常SBM日粮中补充150 mg / kg蛋白酶可增加胰腺相对体重（P <0.05），正常和低SBM日粮中的蛋白酶均会增加肠道相对体重（P <0.01）。较低的SBM降低了胰蛋白酶的活性（P <0.05），而蛋白酶增强了胰蛋白酶的活性（P <0.05）。胰蛋白酶和胰凝乳蛋白酶的活性之间存在SBM水平和蛋白酶水平的相互作用（P <0.01）。总之，在低SBM日粮中补充蛋白酶可以部分补充可消化氨基酸，从而使日粮中的粗蛋白含量降低。
JieYu[a][1];GuixiangYu[a][1][2];BingYu[a];YangZhang[b];JunHe[a];PingZheng[a];XiangbingMao[a];JunqiuLuo[a];ZhiqingHuang[a];YuhengLuo[a];HuiYan[a];QuyuanWang[a];HuifenWang[a];DaiwenChen[a]. Dietary protease improves growth performance and nutrient digestibility in weaned piglets fed diets with different levels of soybean meal[J]. Livestock Science, 2020,241