摘要
Although rare, mycoplasmas are included among the causes of respiratory diseases in reptiles and, in the order Squamata, three reports of these microorganisms causing diseases in pythons have already been reported. This study aimed to evaluate the occurrence of Mycoplasma species in captive snakes. A total of 26 snakes of the families Pythonidae (13), Boidae (7), Viperidae (5) and Colubridae (1) from RioZoo, Brazil, were evaluated. Animals were examined to determine clinical signs consistent with any infectious disease. Tracheal swab samples from snakes were collected in Frey medium and analyzed for the presence of Mycoplasma spp.by isolation and a genus-specific PCR. DNA sequencing analyses of six positive samples by PCR were carried out to identify the species. Using isolation 19.23% (5/26) was positive, while 65.38% (17/26) of the animals were positive by PCR. Based on the analyses of the six sequences obtained, there was similarity with a Mycoplasma spp. previously described in a phyton and, M. agassizii and M. testudineum reported in chelonians. This is the first report of Mycoplasma spp. in animals of the families Boidae and Viperidae. Mycoplasma spp. were detected in snakes with and without clinical signs. The mycoplasmas reported resented identity (range, 95% to 100%) to others already described in reptiles. There was no relationship between the presence of Mycoplasma spp. and clinical signs.
摘要译文
尽管很罕见，但支原体被包括在爬行动物呼吸系统疾病的起因中，并且以鳞翅目（Squamata）的形式，已经报道了三类关于这些微生物在蟒蛇中引起疾病的报告。这项研究旨在评估圈养蛇中支原体物种的发生。对来自巴西里约热内卢的Pythonidae（13），Boidae（7），Viperidae（5）和Colubridae（1）的26条蛇进行了评估。检查动物以确定与任何感染性疾病一致的临床体征。在Frey培养基中收集蛇的气管拭子样品，并通过分离和属特异性PCR分析支原体的存在。通过PCR对六个阳性样品进行DNA测序分析以鉴定该物种。通过PCR分离得到19.23％（5/26）为阳性，而65.38％（17/26）为阳性。基于获得的六个序列的分析，与支原体属物种相似。以前在phyton中有描述，M。agassizii和M. testudineum在chelonians中有报道。这是支原体属的首次报道。在Bo科和Vi科的动物中。支原体在有或没有临床体征的蛇中被发现。支原体报告了与爬行动物中已经描述的其他人相同的身份（范围从95％到100％）。支原体的存在之间没有关系。和临床体征。
MAGALHAES; Barbara Souza Neil et al.. Mycoplasma spp. in captive snakes (Boa constrictor and Bothrops atrox) from Brazil.[J]. Ciencia Rural, 2021,51(1)