期刊文献

Offsprings Produced by Transcervically Inseminating Frozen-thawed Semen into Uterus of a Estrus-induced Saanen Goat during Non-breeding Season 收藏

在非繁殖季节经宫颈受精的冷冻精液进入发情诱导的萨嫩山羊子宫产生的后代
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作者
Yong; Hwan-Yul;Kim; Min-Ah;Bae; Bok-Soo;Kim; Seung-Dong;Jo; Shin-Il;Lim; Yang-Mook;Yoo; Mi-Hyun;Ha; Yong-Hee;Oh; Chang-Shik;Kim; Doo-Hee;
关键词
estrus; frozen-thawed; induction; insemination; Saanen
关键词译文
发情;冻融感应;授精;萨嫩
发布日期
2010.06.30
页码
89-92
来源信息
Journal of Embryo Transfer 한국수정란이식학회지 ISSN：2508-755X, 2010年, 25卷, 2期, 89-92页
摘要
We report herein the successful results of estrus induction, sperm cryopreservation and kids born by transcervical insemination of frozen-thawed semen in a Saanen goat. Flugestone acetate (FGA: 60 mg) was inserted into vagina for 15 days. The goat was intramuscularly injected with 400 IU PMSG and 200 IU hCG (PG600^{(R)} : Intervet, Korea) a day before withdrawal of the FGA sponge. Follicles and corpora lutea were identified on both ovaries by laparoscopy. Artificial insemination was performed 46 hours after removal of FGA sponge. The concentration of frozen-thawed semen was 3.975{\times}10^8/ml 3.975{\times}10^8/ml and 0.5 ml of frozen-thawed semen was transcervically inseminated into uterine body under anesthesia. Three kids, all females, were born 144 days after artificial insemination. This is the first report producing kids by transcervical insemination of frozen-thawed semen in a Saanen goat of which the estrus was induced by FGA sponges, PMSG and hCG during non-breeding season in Korea.
摘要译文
我们在此报告发情诱导，精子冷冻保存和经宫颈授精的萨嫩山羊冷冻精液经宫颈授精所生孩子的成功结果。将醋酸氟石（FGA：60 mg）插入阴道15天。在撤消FGA海绵的前一天，向山羊肌肉注射400 IU PMSG和200 IU hCG（PG600 ^ {（R）}：韩国Intervet）。通过腹腔镜检查在两个卵巢上都鉴定出卵泡和黄体。去除FGA海绵后46小时进行人工授精。冷冻解冻精液的浓度为3.975 {-次} 10 ^ 8 / ml 3.975 {-次} 10 ^ 8 / ml，并在麻醉下经宫颈将0.5ml冷冻解冻精液经子宫授精。人工授精后144天出生了三个孩子，均为雌性。这是在韩国非繁殖季节经宫颈受精的冷冻融化精液通过FGA海绵，PMSG和hCG诱发发情的萨那山羊的第一份报告。
Yong; Hwan-Yul;Kim; Min-Ah;Bae; Bok-Soo;Kim; Seung-Dong;Jo; Shin-Il;Lim; Yang-Mook;Yoo; Mi-Hyun;Ha; Yong-Hee;Oh; Chang-Shik;Kim; Doo-Hee;. Offsprings Produced by Transcervically Inseminating Frozen-thawed Semen into Uterus of a Estrus-induced Saanen Goat during Non-breeding Season[J]. Journal of Embryo Transfer 한국수정란이식학회지, 2010,25(2): 89-92