摘要
Phospholipids, representing 80% of retinal lipids, contain a high level of polyunsaturated fatty acids, which are susceptible to oxidation. It has recently been shown that Folch’ extract of bovine neural retinas reveals moderate photoreactivity by generating reactive oxygen species upon irradiation with blue light. Importantly, the photoreactivity markedly increases upon oxidation of the exctracted lipids. In this work, the phototoxic potential of the Folch’ extract of bovine neural retinas (BRex), subjected to controlled oxidation, is studied in vitro employing a retinal pigment epithelium cell line (ARPE‐19). Multilamellar liposomes prepared from BRex, equilibrated with air, are oxidized in the dark at 37 °C for up to 400 h. The liposomes are then introduced into ARPE‐19 cells by phagocytosis. The impact of BRex at different stages of oxidation on viability and specific phagocytic activity of ARPE‐19 cells exposed to visible light is analyzed. While the oxidized BRex, at the light doses employed, does not induce any measurable killing of the cells, the treatment noticeably decreases their specific phagocytic activity.
摘要译文
代表视网膜脂质的80％的磷脂含有高水平的多不饱和脂肪酸，容易被氧化。最近显示，牛神经视网膜的Folch提取物在蓝光照射下会产生活性氧，从而显示出适度的光反应性。重要的是，一旦提取的脂质氧化，光反应性就会明显增加。在这项工作中，采用视网膜色素上皮细胞系（ARPE-19）在体外研究了经过受控氧化的牛神经视网膜Folch'提取物的光毒性潜能。由BRex制备的多层脂质体经空气平衡后，在黑暗中于37°C氧化长达400°h。然后通过吞噬作用将脂质体引入ARPE-19细胞。分析了BRex在氧化的不同阶段对暴露于可见光下的ARPE-19细胞活力和比吞噬活性的影响。虽然在所用的轻剂量下氧化的BRex不会诱导任何可测量的细胞杀伤，但处理明显降低了它们的比吞噬活性。
Anna M. Pawlak[1];Magdalena Olchawa[1];Anna Koscielniak[1];Andrzej Zadlo[1];Agnieszka Broniec[1];Tomasz Oles[1];Tadeusz J. Sarna[1]. Oxidized Lipids Decrease Phagocytic Activity of ARPE‐19 Cells In Vitro[J]. European Journal of Lipid Science and Technology, 2019,121(9)