摘要
The objective of the present study was to compare the vitrification method for cryopreservation of canine oocytes. Canine cumulus-oocyte complexes (COCs) were collected from ovaries, and were vitrified by ethylene glycol based (E30S) or DMSO based (DAP213) methods. In the E30S method, COCs were exposed to the vitrification solution, composed of 30% ethylene glycol and 0.5 M sucrose, step-wise transferred onto a cryotop holder, then plunged directly into liquid nitrogen. In the DAP213 method, COCs were exposed to 1 M DMSO and DAP213 solution in a cryotube, and thereafter plunged directly into liquid nitrogen. Although vitrified-warmed COCs in the E30S method showed fewer morphological abnormalities, and higher viability than those in the DAP213 method, there was no significant difference in between. These results indicate that either method of vitrification is available and statistically comparable for cryopreservation of canine oocytes.
摘要译文
本研究的目的是比较玻璃化方法冷冻保存犬卵母细胞。从卵巢中收集犬卵-卵母细胞复合物（COCs），并通过基于乙二醇的E30S或基于DMSO的DAP213方法进行玻璃化。在E30S方法中，将COC暴露于玻璃化溶液中，该溶液由30％乙二醇和0.5 M蔗糖组成，逐步转移至冻存架上，然后直接浸入液氮中。在DAP213方法中，将COC暴露于冷冻管中的1 M DMSO和DAP213溶液中，然后直接浸入液氮中。尽管与DAP213方法相比，E30S方法中的玻璃化加热的COC表现出更少的形态学异常，并且具有更高的生存能力，但两者之间没有显着差异。这些结果表明，两种玻璃化方法均可用，并且在冷冻保存犬卵母细胞方面具有统计学可比性。
Yasuyuki Abe[1];Dong-Soo Lee[1];Sang-Keun Kim[2];Hiroshi Suzuki[3]. Vitrification of Canine Oocytes[J]. Journal of Mammalian Ova Research, 2008,25(1): 32-36