摘要
Sperm morphometry is the tool that confers objectivity to the morphological evaluation by accurately measuring the dimensions of the gamete and its structures. Thus, the aim of the study was to perform a morphometric characterization of the domestic cat sperm. Therefore, sperm samples were collected from twenty pairs of epididymis in a TRIS extender at 37oC. An aliquot of the sample was used to make a smear with Rose Bengal solution, and afterwards, the morphology and morphometry were analysed. In the morphology, were quantified the percentage of normal sperm cells, morphological changes of head, midpiece and tail. In morphometry, each normal sperm cell was measured for length, width, area and perimeter of head and midpiece, tail length and total length. The parameters ellipticity, elongation, regularity and rugosity were also determined. The percentage of normal sperm was 67.21%. Of the abnormalities, the curled/folded tail, followed by the curved midpiece, abnormal shaped head and detached head were the most quantified. The sperm head presented 5.56 ± 0.01 μm and 3.10 ± 0.01 μm of length and width, respectively. The head area was 16.94 ± 0.05 μm2, while the perimeter was 16.16 ± 0.03 μm. In the derived parameters, the values were as follows: ellipticity of 1.81 ± 0.00; elongation of 21.39 ± 0.12; regularity of 0.81 ± 0.00; and rugosity of 0.14 ± 0.00. The midpiece presented length and width of 7.96 ± 0.01 μm and 0.76 ± 0.01 μm, respectively. The mean length of the sperm tail was 45.12 ± 0.06 μm, and the total cell size was 58.67 ± 0.06 μm. Thus, it was concluded that the cat sperm is an elongated cell, with high rugosity and regularity. The spermatic tail represents more than ¾ of the total length of the cell and the midpiece exceeds the length of the head.
摘要译文
精子形态计量学是通过精确测量配子的尺寸及其结构赋予客观性的形态评估工具。因此，该研究的目的是对家猫精子进行形态计量学表征。因此，在37oC的TRIS补充剂中从二十对附睾中收集了精子样本。将样品的等分试样用于用玫瑰红溶液涂片，然后分析其形态和形态。在形态学上，定量了正常精子细胞的百分比，头部，中段和尾巴的形态变化。在形态计量学中，测量每个正常精子细胞的长度，宽度，头部和中段的面积和周长，尾巴长度和总长度。还确定了椭圆率，伸长率，规则性和皱纹度的参数。正常精子百分比为67.21％。在异常中，卷曲的/折叠的尾巴，其次是弯曲的中间件，异常形状的头和分离的头是最量化的。精子头的长度和宽度分别为5.56±0.01μm和3.10±0.01μm。头部面积为16.94±0.05μm 2 ，而周长为16.16±0.03μm。在导出的参数中，值如下：椭圆率1.81±0.00；伸长率为21.39±0.12;规律性0.81±0.00;粗糙度为0.14±0.00。中间件的长度和宽度分别为7.96±0.01μm和0.76±0.01μm。精子尾巴的平均长度为45.12±0.06μm，总细胞大小为58.67±0.06μm。因此，可以得出结论，猫的精子是一个细长的细胞，具有很高的皱纹和规则性。精子尾部占细胞总长度的3/4以上，中段超过头部的长度。
Brenna de Sousa Barbosa[1];Herlon Victor Rodrigues Silva[1];Beatriz Evaristo de Almeida Tabosa[1];Thalles Gothardo Pereira Nunes[1];Francisco Felipe de Magalhães[1];Lúcia Daniel Machado da Silva[1]. Morphological and morphometric characterization of domestic cat epididymal sperm[J]. Reproduction in Domestic Animals, 2019,54(12): 1630-1636