期刊文献

Seminal Plasma Anti-Müllerian Hormone: A Potential AI-Boar Fertility Biomarker? 收藏

精浆抗-苗勒管激素：潜在的AI-公猪生育力生物标志物？
原文求助 发布源
作者
Isabel Barranco;Beatriz Fernandez-Fuertes;Lorena Padilla;Ariadna Delgado-Bermúdez;Asta Tvarijonaviciute;Marc Yeste
作者单位
[1] Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, E-17003 Girona, Spain [2] Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, E-17003 Girona, Spain [3] Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Murcia, E-30100 Murcia, Spain [*] Author to whom correspondence should be addressed. Biology 2020, 9(4), 78; https://doi.org/10.3390/biology9040078
关键词
Anti-Müllerian hormone; artificial insemination; fertility; inter-ejaculate variability; seminal plasma; porcine; sperm
关键词译文
抗苗勒氏激素；人工授精；生育能力射精间变异；精浆猪精子
发布日期
2020-04-10
页码
78
DOI
10.3390/biology9040078
来源信息
Biology ISSN：2079-7737, 2020年, 9卷, 4期, 78页
摘要
The anti-Müllerian hormone (AMH), a Sertoli cell-secreted glycoprotein that is present in seminal plasma (SP), is considered as a marker of spermatogenesis in humans. This study aimed to evaluate the presence of this hormone in boar SP, together with its putative relationship with sperm quality, function, and in vivo fertility parameters in liquid-stored semen samples. The concentration of SP-AMH was assessed in 126 ejaculates from artificial insemination (AI)-boars (n = 92) while using a commercial Enzyme-Linked ImmunoSorbent Assay (ELISA) kit with monoclonal antibodies specific for Sus scrofa AMH (CEA228Po, Cloud-clone). Sperm quality (concentration, motility, viability, and acrosome damage) and functionality (membrane lipid disorder and intracellular H2O2 generation) were assessed in semen samples at 0 and 72 h of liquid-storage. In addition, fertility parameters from 3113 sows inseminated with the AI-boars were recorded in terms of farrowing rate, litter size, number of stillbirths per litter, and the duration of pregnancy over a 12-month period. The results revealed that the SP-AMH concentration varied widely among boar ejaculates, with no differences among breeds. Moreover, the SP-AMH concentration proved to be a good predictive biomarker for sperm concentration (p ˂ 0.05), but poor for other sperm quality, functionality, and in vivo fertility parameters of liquid-stored semen samples from AI-boars.
摘要译文
抗苗勒管激素（AMH）是一种存在于精浆（SP）中的支持细胞分泌的糖蛋白，被认为是人类精子发生的标志物。这项研究旨在评估公猪SP中这种激素的存在，以及其与液态储存的精液样本中精子质量，功能和体内生育参数的推定关系。使用商业化酶联免疫吸附测定（ELISA）试剂盒对Sus scrofa AMH特异的单克隆抗体（CEA228Po，Cloud-），用人工授精（AI）-公猪（n = 92）的126批精液评估SP-AMH的浓度。克隆）。在液体储存的0和72 h，评估精液样品的精子质量（浓度，运动性，生存力和顶体损伤）和功能（膜脂质紊乱和细胞内H2O2生成）。此外，还记录了3113头用AI授精的公猪的生育参数，包括分娩率，产仔数，每胎死产数以及12个月内的怀孕时间。结果表明，公猪精液中SP-AMH的浓度差异很大，品种间无差异。此外，SP-AMH浓度被证明是精子浓度的良好预测生物标志物（p˂0.05），但是对于其他精子质量，功能和来自AI公猪的液态精液样品的体内育性参数则较差。
Isabel Barranco;Beatriz Fernandez-Fuertes;Lorena Padilla;Ariadna Delgado-Bermúdez;Asta Tvarijonaviciute;Marc Yeste. Seminal Plasma Anti-Müllerian Hormone: A Potential AI-Boar Fertility Biomarker?[J]. Biology, 2020,9(4): 78