摘要
Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).
摘要译文
克里米亚 - 刚果出血热病毒（CCHFV）是布尼亚病毒科内Nairovirus属，这是普遍的，并导致高死亡率的蜱出生的病毒。 CCHFV的核衣壳包括了由S片段编码的N个蛋白。在这项研究中，N蛋白CCHFV的表达在用重组杆状病毒的昆虫细胞。下的电子显微镜，病毒样颗粒（VLP）与各种大小和形貌进行了观察，在感染的细胞的细胞质囊泡。细胞裂解物的蔗糖梯度纯化表明的VLP主要位于超速离心后的上部分，其通过Western印迹分析和免疫电镜（IEM）确认。
Zhao-rui Zhou [1] Man-li Wang [1] Fei Deng [1] Tian-xian Li [1] Zhi-hong Hu [1] Hua-lin Wang [1]. Production of CCHF virus-like particle by a baculovirus-insect cell expression system[J]. Virologica Sinica, 2011,26(5): 338-346