摘要
The aim of the present study was to examine the effects of fetal bovine serum (FBS) concentration, equilibration time, and oocyte pre-treatment with cytochalasin B (CCB) on subsequent development of vitrified-warmed ovine immature (GVCOCs) and matured (MII) oocytes with (MIICOCs) or without cumulus cells (MIIDOs). In Experiment 1, the effects of FBS concentrations (10 and 20%) during the vitrification-warming procedure were examined. Survival rates after warming were not different between GVCOCs, MIICOCs and MIIDOs oocytes. After in vitro fertilization, rate of cleaved embryos in MIICOCs group at the presence of 20%FBS was higher than MIIDOs and GVCOCs groups. In Experiment 2, the effects of equilibration times (5, 7, and 10 min) were examined. There was no difference in survival rate of vitrified-warmed oocytes equilibrated at different times. Although, the rate of cleavage in MIICOCs and MIIDOs oocytes equilibrated for 10 and 7 min, respectively, was higher than 5 min equilibrated MIIDOs and 7 and 10 min equilibrated GVCOCs oocytes. In Experiment 3, the effects of oocyte pre-treatment with CCB were examined. Despite the insignificant difference in survival rate of vitrified-warmed ovine immature and matured oocytes, the rates of cleavage in CCB pretreated groups were significantly lower than untreated groups. Moreover, the blastocysts were only derived from those cumulus enclosed vitrified-warmed germinal vesicle (GV) and MII oocytes that had been exposed to 10% FBS in the absence of CCB. In conclusion, the presence of cumulus cells, 10% FBS, and the omission of CCB were beneficial for post-warming development of vitrified ovine oocytes.
摘要译文
本研究的目的是研究胎牛血清（FBS）浓度，平衡时间以及用细胞松弛素B（CCB）进行卵母细胞预处理对玻璃化加温绵羊未成熟（GVCOCs）和成熟（MII）后续发育的影响。 ）有（MIICOCs）或没有卵丘细胞（MIIDOs）的卵母细胞。在实验1中，研究了玻璃化-升温过程中FBS浓度（10％和20％）的影响。变温后的存活率在GVCOC，MIICOC和MIIDO卵母细胞之间没有差异。体外受精后，MIICOCs组在20％FBS存在下的卵裂率高于MIIDOs和GVCOCs组。在实验2中，研究了平衡时间（5、7和10分钟）的影响。在不同时间平衡的玻璃化温暖卵母细胞的存活率没有差异。虽然，分别平衡10分钟和7分钟的MIICOC和MIIDOs卵母细胞的切割速率高于平衡5分钟的MIIDO和7和10分钟的GVCOCs卵母细胞。在实验3中，检查了用CCB预处理卵母细胞的效果。尽管玻璃化的，温暖的绵羊未成熟和成熟的卵母细胞的存活率差异不明显，但CCB预处理组的卵裂率明显低于未处理组。此外，胚泡仅来自那些在没有CCB的情况下暴露于10％FBS的封闭的玻璃化温热胚泡（GV）和MII卵母细胞的卵丘。总之，卵丘细胞，10％FBS的存在以及CCB的缺失对于玻璃化羊卵母细胞的升温后发育是有益的。
Abolfazl Shirazi (a1) (a2); Fatemeh Taheri (a2); Hassan Nazari (a2); Maryam Norbakhsh-nia (a2); Ebrahim Ahmadi (a2);Banafsheh Heidari (a3).... Developmental competence of ovine oocyte following vitrification: effect of oocyte developmental stage, cumulus cells, cytoskeleton stabiliser, FBS concentration, and equilibration time[J]. Zygote, 2014,22(2): 165-173