期刊文献

Studies of Hemicellulolytic Enzymes of Bacillus subtilis 收藏

枯草芽孢杆菌半纤维素酶的研究

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作者

Shigenori EMI[1];Juichiro FUKUMOTO[1];Takehiko YAMAMOTO[1]

作者单位

[1]Faculty of Science, Osaka City University

页码

1891-1898

DOI

10.1271/bbb1961.35.1891

来源信息

Agricultural and Biological Chemistry ISSN：0002-1369, 1971年, 35卷, 12期, 1891-1898页

摘要

Many strains of Bacillus subtilis were found to secrete several hemicellulolytic enzymes such as arabinoxylanase, galactomannanase, arabinogalactanase, etc. Chemical and enzy-matic properties of certain strains of the bacterium were comparatively investigated. An arabinogalactanase was purified and obtained in a crystalline state. Its molecular weight and isoelectric point were estimated to be 3.7 ×10⁴ and 8.39, respectively. The enzyme showed an optimal pH for reactions at 6.0, and was stable in a pH range of 5.0 to 9.5 at 30°C. It required no metallic ions for its activity and hydrolyzed soybean arabinogalactan, forming galactobiose as the main product. No liberation of arabinose was observed in the hydrolysate. Also, the enzyme did not attack coffee bean arabinogalactan. Some implications of the experimental results are discussed.

摘要译文

发现许多枯草芽孢杆菌菌株分泌几种半纤维素分解酶，如阿拉伯木聚糖酶，半乳甘露聚糖酶，阿拉伯半乳聚糖酶等。对某些菌株的化学和酶促特性进行了比较研究。纯化阿拉伯半乳聚糖酶并以结晶状态获得。其分子量和等电点估计分别为3.7×10 ^{4 和8.39。该酶在6.0的反应中显示出最佳的pH值，并且在30°C的5.0至9.5的pH范围内稳定。它不需要金属离子即可发挥作用，并水解了大豆阿拉伯半乳聚糖，形成了半乳糖二糖作为主要产品。在水解物中未观察到阿拉伯糖的释放。同样，该酶也没有攻击咖啡豆阿拉伯半乳聚糖。讨论了实验结果的一些含义。}

Shigenori EMI[1];Juichiro FUKUMOTO[1];Takehiko YAMAMOTO[1]. Studies of Hemicellulolytic Enzymes of Bacillus subtilis[J]. Agricultural and Biological Chemistry, 1971,35(12): 1891-1898