摘要
Endogenous adrenocorticotrophic hormone (eACTH) measurement is useful in hypercortisolism and hypoadrenocorticism investigation; however, since the hormone is highly unstable, blood samples require proper processing and storage, as well as shipping is often a step limiting since few laboratories offer this assay in Brazil. The aim of this note was to compare overnight dog′s eACTH preanalytical stability when frozen samples were shipped in dry ice (DI), or with recyclable ice bars (RIB). A total of 56 paired samples for eACTH measurement were analyzed. Blood samples were properly handled, plasma aliquots transferred into plastic microtubes, and stored at -80oC. The fifty-six paired samples were overnight shipped in two thermic isolated boxes with DI or involved by RIB. Despite there was a high correlation between results from both shipping methods (r Spearman = 0.958, P<0.001), the Wilcoxon matched-pairs rank test showed that the shipping method may influence results (P<0.001). However, this difference does not affect results interpretation. By this way, when DI shipping was not possible, RIB shipping may represent a risk to eACTH preanalytical stability.
摘要译文
内源性肾上腺皮质营养激素（eACTH）的测量可用于皮质醇过多症和肾上腺皮质激素缺乏症的研究；但是，由于该激素非常不稳定，因此血液样品需要适当的处理和存储，并且运输通常是一个步骤限制，因为在巴西很少有实验室提供这种检测方法。本文的目的是比较冷冻样品在干冰（DI）或可回收冰块（RIB）中运输时，过夜狗的eACTH分析前稳定性。总共分析了56个用于eACTH测量的配对样品。血液样品经过适当处理，血浆等分试样转移到塑料微管中，并保存在-80oC。五十六个配对的样品在两个带DI的热隔离盒中进行了通宵运输，或由RIB进行了运输。尽管两种运输方法的结果之间具有高度相关性（r Spearman = 0.958，P <0.001），但Wilcoxon配对配对对秩检验表明，运输方法可能会影响结果（P <0.001）。但是，这种差异不会影响结果解释。这样，当无法进行DI运输时，RIB运输可能会对eACTH分析前稳定性造成风险。
POPPL; álan Gomes et al.. Canine endogenous adrenocorticotrophic hormone preanalytical stability after sample shipping in dry ice or recyclable ice bars.[J]. Ciencia Rural, 2021,51(1)