摘要
Crimean-Congo hemorrhagic fever (CCHF) is a severe illness with high fatality. Cases are reported in several countries in Africa, Europe, the Middle East, and Asia. Phylogenetic analyses based on the virus S (nucleocapsid), M (glycoprotein), and L (polymerase) genome segments sequences indicate distinct geographic lineages exist but their specific genetic characteristics require elucidation. In this work we collected all full length S segment sequences and generated a phylogenetic tree based on the alignment of these 62 samples. We then analyzed the alignment using entries from AAIndex, the Amino Acid Index database, to identify amino acid mutations that performed significant changes in charge, pka, hydropathy and side chain volume. Finally, we mapped these changes back to the tree and alignment to identify correlated mutations or sites that characterized a specific lineage. Based on this analysis we are able to propose a number of sites that appear to be important for virus function and which would be good candidates for experimental mutational analysis studies.
摘要译文
克里米亚 - 刚果出血热（CCHF）是一种严重的疾病病死率很高。案件报道的几个国家在非洲，欧洲，中东和亚洲。系统发育分析基于S病毒（核衣壳）上，M（糖蛋白），和L（聚合酶）基因组片段的序列表明不同的地理系存在，但其具体的遗传特性需要澄清。在这项工作中，我们收集了所有的全长S片段的序列和所产生的系统树基于这些62样品的取向。然后，我们分析了使用从AAIndex项的调整，的氨基酸索引数据库，以确定所执行的电荷，PKA，疏水和侧链体积显著改变氨基酸突变。最后，我们映射这些变革回树和排列，以确定相关的突变或网站，表征特定的血统。在此基础上，我们能够提出一些似乎是病毒功能重要，这将是很好的人选实验突变分析研究网站。
Na Han [1] Simon Rayner [1]. Epidemiology and mutational analysis of global strains of crimean-congo haemorrhagic fever virus[J]. Virologica Sinica, 2011,26(4): 229-244