摘要
Oxytocin (OXT) contained in boar semen is known to produce uterine contraction; therefore, we hypothesized that the co-injection of OXT with sperm would improve artificial insemination (AI) using liquid or frozen-thawed boar sperm. We initially examined whether OXT added to semen extender improved sperm transport to the oviduct. Although the addition of OXT did not affect the fresh or frozen-thawed sperm motility or acrosomal integrity, it significantly increased the number of sperm in the oviduct at 6 h after AI injection with OXT, as compared with the control (P < 0.05). Moreover, some sperm were observed in the sperm reservoir of the isthmus in the OXT treatment group, whereas few sperm were observed in the control. When OXT was added to the semen extender immediately prior to AI, the conception rates were significantly higher in both fresh semen and frozen-thawed semen than in the control group (P < 0.05: liquid, 87.5% vs. 70.5%; frozen-thawed, 89.8% vs. 75.0%). From these results, we concluded that the addition of OXT to the semen extender assisted in sperm transportation from the uterus to the oviduct, which resulted in improved reproductive performance.
摘要译文
催产素（OXT）包含在公猪精液是众所周知的产生子宫收缩;因此，我们推测，在共注射OXT与精子将改善人工授精（AI），使用液体或冻融公猪精子。我们初步研究是否OXT加入到精液扩展提高精子的运输到输卵管。尽管添加OXT的没有影响新鲜或冻融精子活力或顶体完整，它显著6增加在输卵管精子数目AI注射OXT h后，与对照组（P而少数精子，观察在控制。当OXT之前被立即添加到精液扩展到AI，构想率显著高于既新鲜精液和冻融精液比对照组（P从这些结果，我们得出结论，除了OXT向精液增量从子宫输卵管，这导致改善的繁殖性能在精子运输协助。
Tetsuji Okazaki[1,†,*]; Erena Ikoma[2,† Tukasa Tinen[3]; Teiichi Akiyoshi[1]; Manabu Mori[1] and Hisanori Teshima[1];. Addition of oxytocin to semen extender improves both sperm transport to the oviduct and conception rates in pigs following AI[J]. Animal Science Journal, 2014,85(1): 8-14