摘要
Bacteria strains isolated from plant leaves of papaya, cassava, sugarcane, yam, and taro, were investigated for their potential probiotic properties. Four isolates from food crop plant leaves were identified as Lactobacillus plantarum, Enterococcus faecalis, Lactobacillus paraplantarum, and Weissella paramesenteroides by sequence of 16S rDNA. The five isolates exhibited tolerance to simulated intestinal juices, and these were selected to evaluate their probiotic properties by biochemical characterization and in vivo testing as a pelleted feed additive. All 5 strains were susceptible to Streptomycin, Penicillin, Chloramphenicol, Ampicillin, and Tetracycline, and exhibited antimicrobial activity against Gram-positive Bacilus cereus, Listeria monocytogenes, Staphylococcus aureus, and the Gram-negative Escherichia coli pathogen. Furthermore, the lactobacilli isolates W. paramesenteroides C02, E. faecalis S04, L. paraplantarum P01, L. plantarum T03, and W. paramesenteroides Y05 exhibited rapid growth in skimmed milk with no nutritional supplement to 108 cfu·mL−1 after 24 h incubation. In vivo analysis of swine feeds after granulation exhibited 109–1010 cfu·kg−1 of L. plantarum, E faecalis, and W. paramesenteroides, which confirmed the probiotic potential and application of the lactobacilli strains isolated from plant leaves in granulated or pelleted feed as additives.
摘要译文
从木瓜，木薯，甘蔗，山药和芋头的植物叶片中分离出的细菌菌株被研究了其潜在的益生菌特性。通过16S rDNA序列鉴定了从粮食作物植物叶片中分离出的四种分离株为植物乳杆菌，粪肠球菌，副植物乳杆菌和副肠韦氏杆菌。这五种分离物对模拟肠液表现出耐受性，并选择这些分离物通过生化表征和作为粒状饲料添加剂的体内测试来评估其益生菌特性。所有5个菌株均对链霉素，青霉素，氯霉素，氨苄青霉素和四环素敏感，并且对革兰氏阳性芽孢杆菌，单核细胞增生李斯特菌，金黄色葡萄球菌和革兰氏阴性大肠杆菌病原体表现出抗菌活性。此外，乳酸杆菌分离株W. paramesenteroides C02，粪肠球菌S04，L。paraplantarum P01，L。plantarum T03和W. paramesenteroides Y05在脱脂牛奶中表现出快速生长，而没有营养补充10 8 培养24小时后，cfu·mL -1 。造粒后猪饲料的体内分析显示植物乳杆菌，粪便和W含量为10 9 –10 10 cfu·kg -1 ….paramesenteroides，证实了益生菌的潜力，并从颗粒状或颗粒状饲料中的植物叶片中分离出的乳杆菌菌株作为添加剂的应用。
LeslySamedi;Albert LintonCharles. Isolation and characterization of potential probiotic Lactobacilli from leaves of food plants for possible additives in pellet feeding[J]. Annals of Agricultural Sciences, 2019,64(1): 55-62