摘要
The aim of this study was to test and compare two new components in extenders for freezing donkey semen: mare colostrum and jenny colostrum. Colostrum was obtained from four mares and four jennies right after the foal's birth. Ejaculates were collected from five fertile donkeys. Sperm samples were pooled, diluted and cryopreserved in three different experimental extender groups: lactose supplemented with egg yolk extender (20%) as the control group, lactose supplemented with jenny colostrum extender (20%), and lactose supplemented with mare colostrum extender (20%). After thawing, we evaluated the sperm motility by means of computer‐assisted analysis, viability by SYBR‐14 and propidium iodide (PI), membrane functional by HOS test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC‐PNA) and PI. The results demonstrated that lactose–jenny colostrum extender displayed significantly higher values (p < .05) in nearly all parameters evaluated – Total Motility, Viability, HOS test, VCL, VSL, VAP, LIN, STR and WOB –, compared with mare colostrum and egg yolk extenders after thawing. In conclusion, the extender containing jenny colostrum used for donkey semen cryopreservation improved the donkey sperm quality after the freezing–thawing process.
摘要译文
这项研究的目的是测试和比较冷冻驴精液补充剂中的两个新成分：母马初乳和珍妮初乳。小马驹出生后，从四匹母马和四只珍妮中获得了初乳。从五只肥沃的驴子中收集射精。在三个不同的实验补充剂组中收集，稀释和冷冻保存精子样品：补充乳糖的鸡蛋黄补充剂（20％）作为对照组，补充詹妮初乳补充剂的乳糖（20％）和补充母乳初乳补充剂的乳糖（20） ％）。解冻后，我们通过计算机辅助分析，SYBR-14和碘化丙啶（PI）评估活力，通过HOS试验检测膜功能以及通过异硫氰酸酯与花生凝集素（FITC-PNA）和PI结合来评估顶体完整性。结果表明，与母乳初乳相比，乳糖-珍妮初乳增量剂在几乎所有评估的参数（总运动性，生存力，HOS测试，VCL，VSL，VAP，LIN，STR和WOB）中均显示出明显更高的值（p <.05）。和蛋黄补充剂解冻后。总之，用于冷冻驴精液的含有珍妮初乳的补充剂可提高冷冻解冻后驴精子的质量。
Cristina álvarez[1];Noelia González[2];Victoria Luño[2];Felisa Martínez[2];Lydia Gil[2]. Alternatives in Donkey semen cryopreservation: Mare vs. Jenny Colostrum[J]. Reproduction in Domestic Animals, 2019,54(Sup4): 94-97