摘要
This study aimed to evaluate the effects of a combination of feed additives with complementary functional properties on the intestinal microbiota, homocysteine, and vitamins E and B status as well as systemic immune response of weanling piglets. At weaning, 32 litters were assigned to one of the following dietary treatments (DT): 1) conventional diet (CTRL); 2) CTRL diet supplemented with antibiotics (ATB); 3) a cocktail of feed additives containing cranberry extract, encapsulated carvacrol, yeast-derived products, and extra vitamins A, D, E, and B complex (CKTL); or 4) CKTL diet with bovine colostrum in replacement of plasma proteins (CKTL + COL). Within each litter, the piglets with lowest and highest birth weights (LBW and HBW, respectively) and two piglets of medium birth weight (MBW) were identified. The MBW piglets were euthanized at 42 d of age in order to characterize the ileal and colonic microbiota. Blood samples were also collected at weaning and at 42 d of age from LBW and HBW piglets to measure insulin-like growth factor-1 (IGF-1), cysteine, homocysteine, and vitamins E, B6, and B12, and to characterize the leukocyte populations. At 42 d of age, cytokine production by stimulated peripheral blood mononuclear cells was also measured. In a second experiment, piglets were reared under commercial conditions to evaluate the effects of the DT on the growth performance. At the indicator species analysis, the highest indicator value (IV) for Succinivibrio dextrinosolvens was found in the CKTL group, whereas the highest IV for Lactobacillus reuteri and Faecalibacterium prausnitzii was evidenced in the CKTL + COL group (P P P P
摘要译文
这项研究旨在评估具有互补功能特性的饲料添加剂组合对断奶仔猪肠道微生物群，同型半胱氨酸以及维生素E和B状态以及全身免疫反应的影响。断奶时，将32窝垃圾分配给以下一种饮食疗法（DT）：1）常规饮食（CTRL）； 2）CTRL饮食补充抗生素（ATB）； 3）饲料添加剂的混合物，包含蔓越莓提取物，胶囊化的香芹酚，酵母衍生产品以及额外的维生素A，D，E和B复合物（CKTL）；或4）CKTL饮食配合牛初乳替代血浆蛋白（CKTL + COL）。在每个窝中，鉴定出出生体重最低和最高的仔猪（分别为LBW和HBW）和两个中等出生体重的仔猪（MBW）。为了鉴定回肠和结肠菌群，在42 d处对MBW仔猪实施安乐死。在断奶时和42 d龄时从LBW和HBW仔猪处采集血样，以测量胰岛素样生长因子-1（IGF-1），半胱氨酸，高半胱氨酸和维生素E，B 6和B 12以及表征白细胞种群。在42岁时，还测量了受刺激的外周血单核细胞产生的细胞因子。在第二个实验中，在商业条件下饲养仔猪，以评估DT对生长性能的影响。在指示物种类分析中，CKTL组中发现了琥珀酸葡聚糖溶解酶的最高指示值（IV），而CKTL + COL组中的罗伊氏乳杆菌和普氏嗜热杆菌的IV最高（P P P P
Luca Lo Verso [1]; Guylaine Talbot [2]; Bruno Morissette [2]; Frédéric Guay [1]; J Jacques Matte [2]; Chantal Farmer [2]; Joshua Gong [3]; Qi Wang [3]; Nathalie Bissonnette [2]; Carole Beaulieu [4]; Martin Lessard [2]. The combination of nutraceuticals and functional feeds as additives modulates gut microbiota and blood markers associated with immune response and health in weanling piglets[J]. Journal of Animal Science, 2020,98(8)