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Influence of diet shift from bloodworm to formulated feed on growth performance, gut microbiota structure and function in early juvenile stages of hybrid sturgeon (Acipenser baerii × Acipenser schrenckii) 收藏

禽类转移从嗜鼻的影响对增殖性能，肠道微生物群结构和杂交鲟的早期阶段的功能（Acipenser Baerii×Acipenser Schrenckii）
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作者
QiangHao[a];TsegayTeame[a][b];XuexiangWu[c];QianwenDing[d];ChaoRan[e];YalinYang[e];YuenanXing[f]
作者单位
[a]aChina-Norway Joint Lab on Fish Gastrointestinal Microbiota, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaChina-Norway Joint Lab on Fish Gastrointestinal Microbiota, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China[b]bTigray Agricultural Research Institute, Tigray, Mekelle, EthiopiaTigray Agricultural Research Institute, Tigray, Mekelle, Ethiopia[c]cGuizhou University, Guiyang 550025, ChinaGuizhou University, Guiyang, 550025, China[d]dNorway-China Joint Lab on Fish Gastrointestinal Microbiota, Institute of Biology, Norwegian University of Science and Technology, Trondheim, NorwayNorway-China Joint Lab on Fish Gastrointestinal Microbiota, Institute of Biology, Norwegian University of Science and Technology, Trondheim, Norway[e]eKey Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China[f]fLiaoning Provincial Key Laboratory of Zoonosis, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaLiaoning Provincial Key Laboratory of Zoonosis, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China[鈦�]鈦�
关键词
Bloodworm;Formulated feed;Gut microbiota;Sturgeon;Germ free zebrafish model;
关键词译文
血虫;配制饲料;肠道微生物肿;鲟鱼;胚芽斑马鱼模型;
发布日期
25 February 2021
DOI
10.1016/j.aquaculture.2020.736165
来源信息
Aquaculture ISSN：0044-8486, 2021年, 533卷
摘要
Currently sturgeon farming is one of the common cold fresh water farming in China, but its production is very low comparing with the country's cold water fish farming potential. One of the main reasons for the low production of sturgeon is unavailability of proper feed for early live stage of sturgeon. The aim of this study was to examine the effects of diet shift from live food (bloodworm) to formulated feed on growth performance, structure and function of gut microbiota in early juvenile stages of hybrid sturgeon (Acipenser baerii 脳 Acipenser schrenckii). Significantly increased in weight gain rate (WGR) and feed conversion ratio (FCR) were observed in the sturgeon group fed on the formulated versus the bloodworm group (p聽<聽0.05). Significantly higher Proteobacteria was recorded in the formulated feed group (p聽<聽0.05), while Fusobacteria was significantly increased in the bloodworm group (p聽<聽0.05). Furthermore, effects of colonization of the gut microbiota isolated from each group of sturgeon on host immunity was evaluated using germ free (GF) zebrafish model. The expression of genes related to anti-inflammatory (IL-10, TGF-尾), non-specific immune (Lysozyme, C3a), and anti-virus (IFN桅3, Mxb, Mxc) were significantly decreased in GF zebrafish colonized with gut microbiota of sturgeon fed on formulated feed, while expression of pro-inflammatory gene (TNF伪) was significantly increased in formulated feed group. Moreover, GF zebrafish colonized with gut microbiota of sturgeon fed on the formulated feed revealed significantly lower survival rate for A. veronii Hm091 challenge (p聽<聽0.05). In conclusion, the present study revealed that, compared with bloodworm, the formulated feed enhanced the WGR and FCR of sturgeon, but induced intestinal microbiota dysbiosis, which negatively affected the immune response of GF zebrafish and decreased their resistance to pathogenic bacteria.
摘要译文
目前Sturgeon农业是中国普通冷的淡水养殖之一，但其生产与国家冷水鱼养殖潜力相比非常低。低生产鲟鱼的主要原因之一是鲟鱼早期活阶段的适当饲料的不可用。本研究的目的是研究饮食从活食物（血虫）的效果，以在杂交鲟（Acipenser Baerii脳acipenserschrenii）的早期幼年阶段进行肠道微生物的生长性能，结构和功能饲料。体重增加率（WGR）显着增加，在配制的与血虫组（P <0.05）喂食的鲟鱼组中观察到饲料转化率（FCR）。在配制的进料组中记录了显着较高的植物菌（P <0.05），而血虫组（P <0.05）中血管菌明显增加。此外，使用胚芽（GF）斑马鱼模型评估从每组鲟鱼中分离的肠道微生物的肠道微生物的疗效。在GF斑马皮法殖民化中，与抗炎（IL-10，TGF-），非特异性免疫（溶菌酶，C3A），非特异性免疫（溶菌酶，C3A）和抗病毒（IFN 3，MXB，MXC）的表达显着降低肠道鲟饲喂配合饲料的微生物群，而促炎性基因（TNF伪）的表达在配合饲料组显著增加。此外，GF斑马鱼与配方饲料饲喂鲟鱼的肠道微生物殖民殖民显示出对A.Veronii HM091攻击的显着降低的存活率（P <0.05）。总之，本研究表明，与醋虫相比，配制的饲料增强了鲟鱼的WGR和FCR，但诱导的肠道微生物菌症，这对GF斑马鱼的免疫应答产生了负面影响，并降低其对致病细菌的抗性。
QiangHao[a];TsegayTeame[a][b];XuexiangWu[c];QianwenDing[d];ChaoRan[e];YalinYang[e];YuenanXing[f]. Influence of diet shift from bloodworm to formulated feed on growth performance, gut microbiota structure and function in early juvenile stages of hybrid sturgeon (Acipenser baerii × Acipenser schrenckii)[J]. Aquaculture, 2021,533