摘要
This study was carried out to examine on developmental competence of Hanwoo embryos cultured in vitro according to culture conditions and freezing methods. The in vitro developmental competence to blastocyst stage at Day 8 of culture in SOF was significantly (p<0.05) higher than that in CR1aa (30.3% vs. 18.4%). The in vitro developmental rate of morula and blastocysts cultured in group culture was significantly (p<0.05) higher than that in individual culture (41.4% and 36.0% vs. 21.1% and 10.5%, respectively). The cell number of Day 8 blastocysts in group culture was significantly (p<0.05) higher than that in the individual culture ($120.1{\pm}12.8\;vs.\;94.1{\pm}12.1$, respectively). The survival rates of frozen-thawed balstocysts that were exposed in 1.5 M ethylene glycol or 1.5 M ethylene glycol containing 0.1 M sucrose were 77.5% and 78.7%, respectively. The survival rates of blastocysts cultured for 48 h in slow freezing and vitrification was not significantly different (73.3 and 74.0%). In conclusion, in vitro developmental competence of bovine embryos was influenced on the culture medium (SOF) and culture method (Group culture). Survival rate of frozen-thawed of bovine embryos was not influenced on freezing solutions and freezing methods.
摘要译文
根据培养条件和冷冻方法，研究了体外培养的韩宇胚胎的发育能力。 SOF培养第8天对胚泡期的体外发育能力显着（p <0.05）高于CR1aa（30.3％对18.4％）。组培养的桑mor和胚泡的体外发育率显着（p <0.05）高于单个培养的（分别为41.4％和36.0％，分别为21.1％和10.5％）。组培养的第8天胚泡的细胞数显着（p <0.05）比个体培养的细胞数高（分别为$ 120.1 {-pm} 12.8-; vs .-; 94.1 {-pm} 12.1 $）。暴露于含有0.1 M蔗糖的1.5 M乙二醇或1.5 M乙二醇中的冻融的囊肿的存活率分别为77.5％和78.7％。缓慢冷冻和玻璃化培养48 h的胚泡存活率没有显着差异（73.3％和74.0％）。总之，牛胚胎的体外发育能力受培养基（SOF）和培养方法（分组培养）的影响。牛胚胎冻融后的存活率不受冻液和冷冻方法的影响。
Song; S.H;Min; C.S;Son; G.D;Rho; C.W;Kang; Y.S;Park; C.S;Kong; I.K;. Effect of Culture Conditions and Freezing Methods on Developmental Competence of Hanwoo Embryos Cultured In Vitro[J]. Journal of Embryo Transfer 한국수정란이식학회지, 2007,22(4): 251-255