摘要
In the present study, we aimed to evaluate the effects of different concentrations of selenium (Se) ovine nutritional supplementation on spermatozoa DNA integrity. Thirty male ovines (age: 10 months) were used. They were fed with hay and ram food in an intensive system, which was divided into stalls (5 m long and 3 m wide) with feeding troughs, and had ad libitum access to food and water. Ovines in group 1 (G1, the negative control) received mineral salt supplementation without Se; ovines in G2 received the same mineral salt mixed with 5 mg Se (as sodium selenite)/kg mineral supplement;ovines in G3 received 10 mg Se/kg mineral supplement; ovines in G4 received 15 mg Se/kg mineral supplement; and ovines in G5 received 20 mg Se/kg mineral supplement. Ovines in all groups remained untreated for 14 days, followed by a treatment period of 56 days. Semen samples were obtained by electroejaculation. The DNA damage in semen samples was evaluated using the comet assay. The experimental design was implemented using a 5 × 5 Latin Square, i.e., five treatments and five experimental periods. The mean differences were compared using Tukey’s test at a significance level of 5%. The control group (G1) showed a high percentage of DNA damage compared to the Se-treated groups (G2-G5). Therefore, Se supplementation could decrease the basal level of DNA damage in sperm cells, suggesting that Se might exert protective effects on sperm DNA.
摘要译文
在本研究中，我们旨在评估不同浓度的硒（Se）绵羊营养补充剂对精子DNA完整性的影响。使用了30只雄性绵羊（年龄：10个月）。他们在集约化的系统中饲喂干草和公羊食物，该系统分为五个摊位（长5 m，宽3 m），带有饲槽，可以随意获取食物和水。第一组（G1，阴性对照）的绵羊接受了不含硒的矿物质盐补充； G2中的绵羊接受相同的矿物质盐，并添加5 mg Se（作为亚硒酸钠）/ kg矿物质补充剂； G3中的绵羊接受10 mg硒/ kg矿物质补充剂； G4中的绵羊接受了15 mg Se / kg矿物质补充剂； G5中的绵羊接受了20 mg Se / kg矿物质补充。所有组中的绵羊均未治疗14天，随后为56天的治疗期。通过电射精获得精液样品。使用彗星试验评估精液样本中的DNA损伤。实验设计是使用5×5的拉丁方进行的，即五次治疗和五个实验时期。使用Tukey检验以5％的显着性水平比较了平均差异。对照组（G1）与硒处理组（G2-G5）相比，DNA损伤率高。因此，添加硒可以降低精子细胞DNA损伤的基础水平，表明硒可能对精子DNA有保护作用。
MOYA; Carla Fredrichsen et al.. Selenium supplementation prevents DNA damage in ram spermatozoa.[J]. Ciencia Rural, 2021,51(1)