摘要
Autism spectrum disorder (ASD) has a high incidence of intestinal comorbidity, indicating a strong association with gut microbiota. The purpose of this study was to characterize gut microbiota profiles in children with ASD. Seventy-seven children with ASD [33 with mild ASD and 44 with severe ASD according to the Childhood Autism Rating Scale score] and 50 age-matched healthy children were enrolled. Compared with children in the healthy control (HC) group, those in the ASD group showed higher biomass, richness, and biodiversity of gut microbiota, and an altered microbial community structure. At the genus level, there was a significant increase in the relative abundance of unidentified Lachnospiraceae, Clostridiales, Erysipelotrichaceae, Dorea, Collinsella, and Lachnoclostridium, whereas Bacteroides, Faecalibacterium, Parasutterella, and Paraprevotella were significantly lower in the ASD group than in the control group. The presence of unidentified Erysipelotrichaceae, Faecalibacterium, and Lachnospiraceae was positively correlated with ASD severity. Notably, three microbial markers (Faecalitalea, Caproiciproducens and Collinsella) were identified in a random forest model with an area under the curve (AUC) of 0.94 for differentiation between HCs and ASD patients. Furthermore, the validation model was consistent with the discovery set (AUC = 0.98, 95% CI: 97.9%–100%). The training and testing sets were more effective when the number of bacteria was increased. In addition, the functional properties (such as galactose metabolism, glycosyltransferase activity, and glutathione metabolism) displayed significant differences between the ASD and HC groups. The current study provides evidence for the relationship between gut microbiota and ASD, with the findings suggesting that gut microbiota could contribute to symptomology. Thus, modulation of gut microbiota may be a new therapeutic strategy for ASD.
摘要译文
自闭症谱系障碍（ASD）的肠道合并症发生率很高，表明它与肠道菌群密切相关。这项研究的目的是表征ASD儿童的肠道菌群特征。入选了70例ASD患儿（根据儿童自闭症评分量表得分为33例轻度ASD和44例重度ASD）和50个年龄相匹配的健康儿童。与健康对照组相比，ASD组的肠道菌群具有更高的生物量，丰富性和生物多样性，并且微生物群落结构发生了变化。在属水平上，未鉴定的鞭毛螺旋藻科，梭菌，梭菌，杜蕾，Collinsella和梭状梭菌的相对丰度显着增加，而ASD组中的拟杆菌，Faecalibacterium，Parasutterella和Paraprevotella则明显低于对照组。 。身份不明的紫锥菊科，Faecalibacterium和Lachnospiraceae的存在与ASD严重程度呈正相关。值得注意的是，在随机森林模型中鉴定出了三种微生物标记（Faecalitalea，Caproiciproducens和Collinsella），曲线下面积（AUC）为0.94，用于区分HCs和ASD患者。此外，验证模型与发现集一致（AUC = 0.98，95％CI：97.9％–100％）。当细菌数量增加时，训练和测试集更有效。此外，ASD组和HC组之间的功能特性（例如半乳糖代谢，糖基转移酶活性和谷胱甘肽代谢）表现出显着差异。当前的研究为肠道菌群与ASD之间的关系提供了证据，研究结果表明肠道菌群可能与症状有关。因此，调节肠道菌群可能是ASD的新治疗策略。
XueDing[a][1];YiranXu[a][1];XiaoliZhang[a];LinglingZhang[a];GuiqinDuan[b];ChunlanSong[b];ZhenghuaLi[b];YanyanYang[b];YazheWang[b];XiaoyangWang[a][c];ChanglianZhu[a][d][e]. Gut microbiota changes in patients with autism spectrum disorders[J]. Journal of Psychiatric Research, 2020,129: 149-159