摘要
As a protein expression vector, the baculovirus demonstrates many advantages over other vectors. With the development of biotechnology, baculoviral vectors have been genetically modified to facilitate high level expression of heterologous proteins in both insect and mammalian cells. These modifications include utilization of different promoters and signal peptides, deletion or replacement of viral genes for increasing protein secretion, integration of polycistronic expression cassette for producing protein complexes, and baculovirus pseudotyping, promoter accommodation or surface display for enhancing mammalian cell targeting gene delivery. This review summarizes the development and the current state of art of the baculovirus expression system. Further development of baculovirus expression systems will make them even more feasible and accessible for advanced applications.
摘要译文
作为蛋白质表达载体，杆状病毒显示了过度的其它载体的许多优点。随着生物技术的发展，杆状病毒载体已被遗传修饰，以促进在昆虫和哺乳动物细胞的异源蛋白质的高水平表达。这些修改包括利用不同的启动子和信号肽，缺失或替换病毒基因用于提高蛋白质的分泌，融合用于产生蛋白质复合多顺反子表达盒，和杆状病毒假型，启动子住宿或表面展示用于增强靶向基因递送哺乳动物细胞的。本文总结了发展和艺术杆状病毒表达系统的当前状态。杆状病毒表达系统的进一步发展将令他们更加可行和可访问的先进应用。
Shu-fen Li [1] Hua-lin Wang [1] Zhi-hong Hu [1] Fei Deng [1]. Genetic modification of baculovirus expression vectors[J]. Virologica Sinica, 2012,27(2): 71-82