摘要
Rumen cannulation is a widely employed technique in ruminant nutrition research. However, the gap between skin and rumen cannula can cause leakage of fermentation gases and influx of atmospheric air, which may adversely affect the anaerobic environment in the rumen. The present study was designed to investigate the effects of rumen cannulation on headspace gases, dissolved gases, fermentation end products, and methanogen community in the rumen of dairy cows. Eight Holstein cows were used in the experiment. Four cows were surgically fitted with rumen cannulas, whereas the other 4 intact cows were used as control. Rumen cannulation decreased gaseous hydrogen and methane concentrations, dissolved carbon dioxide concentration, and relative abundances of Methanosphaera, and increased the saturation factor of dissolved hydrogen and dissolved methane, dissolved methane concentration, volatile fatty acid concentration, 16S ribosomal RNA gene copies of methanogens, and Simpson index of methanogen community. In summary, rumen cannulation causes a reduction in headspace gaseous hydrogen and gaseous methane, which may not decrease dissolved gas concentrations due to an increase in saturation factors. Furthermore, rumen cannulation alters methanogen community with increased methanogen population and decreased relative abundances of Methanosphaera.
摘要译文
瘤胃插管是反刍动物营养研究中广泛使用的技术。但是，皮肤和瘤胃套管之间的间隙会导致发酵气体泄漏和大气流入，这可能对瘤胃中的厌氧环境产生不利影响。本研究旨在调查瘤胃插管对奶牛瘤胃顶空气体，溶解气体，发酵终产物和产甲烷菌群落的影响。实验中使用了八头荷斯坦奶牛。通过外科手术使四头母牛装有瘤胃插管，而其他四头完整的母牛用作对照。瘤胃插管降低了气态氢和甲烷的浓度，溶解的二氧化碳的浓度以及甲烷菌的相对丰度，并增加了溶解氢和溶解的甲烷的饱和因子，溶解的甲烷的浓度，挥发性脂肪酸的浓度，产甲烷菌的16S核糖体RNA基因拷贝以及产甲烷菌群落的辛普森指数。总之，瘤胃插管导致顶部空间气态氢和气态甲烷的减少，由于饱和因子的增加，这可能不会降低溶解气体的浓度。此外，瘤胃插管改变了产甲烷菌的群落，增加了产甲烷菌的数量，降低了产甲烷球菌的相对丰度。
RongWang[1][2];MinWang[2][3];Xiu MinZhang[2];Jiang NanWen[1][2];Zhi YuanMa[2];Dong LeiLong[1][2];Jin PingDeng[1][4];Zhi LiangTan[2][3];. Effects of rumen cannulation on dissolved gases and methanogen community in dairy cows[J]. Journal of Dairy Science, 2019,102(3): 2275-2282