摘要
A complement fixation (CF) test for the diagnosis of Trypanosome congolense infection in cattle was developed and compared with the indirect fluorescent antibody (IFA) test.
Serological investigations were made with cattle immunized with irradiated T. congolense and challenged with untreated T. congolense. Trypanosomal antibodies were demonstrated by the CF test. The results indicated good specificity and sensitivity between the CF method and the IFA test. The CF test also afforded easier reading of results than the IFA test; however, antigen preparation was more difficult in the former.
The serological responses detected by the IFA and CF test appeared to be influenced by early variations of parasitemia, although no correlation appeared to exist between titer and parasitemia at later stages of the infection. Antibodies were detected in animals which received injections of irradiated T. congolense prior to challenge with viable organisms.
摘要译文
开发了用于诊断牛中锥虫病虫感染的补体固定（CF）试验，并与间接荧光抗体（IFA）试验进行了比较。用经辐照的刚果聚糖（T. congolense）免疫的牛进行血清学调查，并用未处理的刚果蝇（T. congolense）攻击。通过CF测试证明了锥虫体抗体。结果表明CF方法和IFA测试之间具有良好的特异性和灵敏度。 CF测试也比IFA测试更容易阅读结果;然而，前者的抗原制备更加困难。通过IFA和CF检测检测到的血清学反应似乎受到寄生虫血症的早期变化的影响，尽管在感染的后期阶段滴度和寄生虫血症之间似乎不存在相关性。在用活生物体攻击之前接受注射辐射的刚果红单胞菌的动物中检测到抗体。
R. Lötzsch. Trypanosoma congolense[J]. Experimental Parasitology, 1974,36(1): 27-33