摘要
Common aspects of infertility can be seen across several species. In humans, dairy cows, and mares there is only a 25-35% chance of producing a live offspring after a single insemination, whether natural or artificial. Oocyte quality and subsequent embryo development can be affected by factors such as nutrition, hormonal regulation, and environmental influence. The objective of this study was to identify genes expressed in oocytes and/or cumulus cells, across a diverse range of species, which may be linked to the ability an oocyte has to develop following fertilization. Performing a meta-analysis on previously published microarray data on various models of oocyte and embryo quality allowed for the identification of 56 candidate genes associated with oocyte quality across several species, 4 of which were identified in the cumulus cells that surround the oocyte. Twenty-one potential biomarkers were associated with increased competence and 35 potential biomarkers were associated with decreased competence. The upregulation of Metap2, and the decrease of multiple genes linked to mRNA and protein synthesis in models of competence, highlights the importance of de novo protein synthesis and its regulation for successful oocyte maturation and subsequent development. The negative regulation of Wnt signaling has emerged in human, monkey, bovine, and mouse models of oocyte competence. Atrx expression was linked to decreased competence in both oocytes and cumulus cells. Biological networks and transcription factor regulation associated with increased and decreased competence were also identified. These genes could potentially act as biomarkers of oocyte quality or as pharmacological targets for manipulation in order to improve oocyte developmental potential.
摘要译文
几个物种可以看到不育症的常见方面。在人类，奶牛和母马中，只有25-35％的机会在单次授精后产生活的后代，无论是天然的还是人工的。卵母细胞质量和随后的胚胎发育可能受到诸如营养，激素调节和环境影响等因素的影响。这项研究的目的是确定卵母细胞和/或卵丘细胞中表达的基因，涵盖多种物种，这可能与受精后卵母细胞发育的能力有关。对以前发表的关于卵母细胞和胚胎质量模型的微阵列数据进行荟萃分析，可以鉴定出56个与卵母细胞质量相关的候选基因，其中4个在卵母细胞周围的卵丘细胞中被鉴定出来。 21种潜在的生物标志物与能力提高有关，35种潜在的生物标志物与能力下降有关。 Metap2的上调，以及与能力模型中mRNA和蛋白质合成相关的多个基因的减少，突出了从头蛋白质合成及其对成功卵母细胞成熟和随后发育的调节的重要性。 Wnt信号传导的负调节已经在人，猴，牛和小鼠卵母细胞能力模型中出现。 Atrx表达与卵母细胞和卵丘细胞的能力降低有关。还鉴定了与增加和降低的能力相关的生物网络和转录因子调节。这些基因可能潜在地充当卵母细胞质量的生物标志物或作为操纵的药理学靶标以改善卵母细胞发育潜力。
Lynne C. O'Shea[1];Jai Mehta[2];Patrick Lonergan[2];Carmel Hensey[1];Trudee Fair[3]. Developmental competence in oocytes and cumulus cells: candidate genes and networks[J]. Archives of Andrology, 2012,58(2): 88-101