摘要
The aim of this study was to screen potential probiotic lactic acid bacteria from Chinese spontaneously fermented non-dairy foods by evaluating their probiotic and safety properties. All lactic acid bacteria (LAB) strains were identified by 16S rRNA gene sequencing. The in vitro probiotic tests included survival under low pH and bile salts, cell surface hydrophobicity, auto-aggregation, co-aggregation, antibacterial activity, and adherence ability to cells. The safety properties were evaluated based on hemolytic activity and antibiotic resistance profile. The salt tolerance, growth in litmus milk, and acidification ability were examined on selected potential probiotic LAB strains to investigate their potential use in food fermentation. A total of 122 strains were isolated and identified at the species level by 16S rRNA gene sequencing and included 62 Lactobacillus plantarum, 40 Weissella cibaria, 12 Lactobacillus brevis, 6 Weissella confusa, and 2 Lactobacillus sakei strains. One W. cibaria and nine L. plantarum isolates were selected based on their tolerance to low pH and bile salts. The hydrophobicity, auto-aggregation, co-aggregation, and antagonistic activities of these isolates varied greatly. All of the 10 selected strains showed multiple antibiotic resistance phenotypes and no hemolytic activity. The highest adhesion capacity to SW480 cells was observed with L. plantarum SK1. The isolates L. plantarum SK1, CB9, and CB10 were the most similar strains to Lactobacillus rhamnosus GG and selected for their high salt tolerance and acidifying activity. The results revealed strain-specific probiotic properties were and potential probiotics that can be used in the food industry.
摘要译文
本研究的目的是通过评估其益生菌和安全性来筛选来自中国自发发酵的非乳制品的潜在益生菌乳酸菌。通过16S rRNA基因测序鉴定所有乳酸菌（LAB）菌株。体外益生菌测试包括在低pH和胆汁盐下的存活，细胞表面疏水性，自动聚集，共聚集，抗细菌活性和对细胞的粘附能力。基于溶血活性和抗生素抗性谱评估安全性质。在选择的潜在益生菌LAB菌株上检查盐耐受性，石蕊乳中的生长和酸化能力，以研究它们在食品发酵中的潜在用途。通过16S rRNA基因测序在物种水平上分离和鉴定了总共122个菌株，包括62株植物乳杆菌，40个Weissella cibaria，12个短乳杆菌，6个Weissella confusa和2个Lactobacillus sakei菌株。基于它们对低pH和胆汁盐的耐受性选择一种W.cibaria和九种植物乳杆菌分离物。这些分离物的疏水性，自聚集，共聚集和拮抗活性变化很大。所有10种选择的菌株均显示出多种抗生素抗性表型并且没有溶血活性。用植物乳杆菌SK1观察到对SW480细胞的最高粘附能力。分离株植物乳杆菌SK1，CB9和CB10是与鼠李糖乳杆菌GG最相似的菌株，并因其高盐耐受性和酸化活性而被选择。结果显示菌株特异性益生菌特性和可用于食品工业的潜在益生菌。
Jing Wang[1]]Jing Wang[1]]Kun Yang[1]]Miaomiao Liu[1]]Jie Zhang[1]]Xinyuan Wei[1]]Mingtao Fan[1]]. Screening for potential probiotic from spontaneously fermented non-dairy foods based on in vitro probiotic and safety properties[J]. Annals of Microbiology, 2018,68(12): 803–813