摘要
In sheep industry, genetic progress rate achieved by artificial insemination (AI) is limited by the convoluted anatomy of the cervix, which does not allow the passage of an insemination catheter for uterine semen deposition. The aim of this study was to test, in 98 pregnant at term Sarda ewes, the effects of: Experiment 1) total or partial ablation of cervical folds and Experiment 2) 4 or 2 incisions of cervical folds, on the passage of an insemination catheter, deposition of frozen-thawed semen and pregnancy rates. Surgical procedures were performed within 24 h from parturition providing deep sedation and epidural anaesthesia. Duration of surgeries and post-operatory recovery were carefully monitored. For both experiments, 5 months since surgery, independently of the stage of oestrus cycle, cervical patency was tested through the transcervical passage of a palpation probe. Six months since surgery, in Experiment 1, ewes were naturally mated with fertile rams. In Experiment 2, ewes submitted to incisions of the cervical folds and a control group underwent synchronisation of oestrus and transcervical AI with frozen-thawed semen. Thirty days later, for both experiments, pregnancy rates were assessed by ultrasonography and lambing rates were recorded. Five months after surgery, in Experiment 1, transcervical passage of a palpation probe to reach the uterine lumen was possible in all ewes submitted to total and partial ablation of folds. In Experiment 2, this was achievable in 90.5% ewes with 4 incisions of the folds and in 89.6% ewes with 2 incisions with no significant differences among groups (P = 0.44). In Experiment 1, pregnancy rates in ewes mated to rams after total or partial ablation of the cervical folds was 100%. In Experiment 2, following transcervical AI, pregnancy rates were higher in groups submitted to 4 (63.7%) or 2 (41.4%) incisions of the cervical folds compared to the control group (8%; P
摘要译文
在绵羊产业中，通过人工授精（AI）实现的遗传进展速率受到子宫颈的回旋解剖结构的限制，其不允许用于子宫精液沉积的授精导管通过。这项研究的目的是在怀孕期间测试98例Sarda母羊的影响：实验1）颈部褶皱的全部或部分消融和实验2）4或2个颈部褶皱切口，在授精导管通过，冷冻解冻精液的沉积和怀孕率。在分娩后24小时内进行外科手术，提供深度镇静和硬膜外麻醉。仔细监测手术持续时间和术后恢复情况。对于两个实验，自手术后5个月，与发情周期无关，通过触诊探针的经宫颈传代测试宫颈通畅。手术后6个月，在实验1中，母羊与肥沃的公羊自然交配。在实验2中，提交到颈部褶皱切口的母羊和对照组经历了发情和经宫颈AI与冷冻 - 解冻精液的同步。三十天后，对于两个实验，通过超声检查评估怀孕率并记录产羔率。在手术后5个月，在实验1中，在所有接受全部和部分消融褶皱的母羊中，触诊探针到达子宫腔的经宫颈传导是可能的。在实验2中，这可以在90.5％的母羊中实现，其中4个褶皱切口和89.6％的母羊具有2个切口，组间没有显着差异（P = 0.44）。在实验1中，在全部或部分消融宫颈褶皱后与母羊交配的母羊的妊娠率为100％。在实验2中，在经宫颈AI后，与对照组相比，接受4次（63.7％）或2次（41.4％）颈部切口切除的组的妊娠率更高（8％; P
SalvatorePau[a][b];LauraFalchi[a];MauroLedda[a];LuisaBogliolo[a];FedericaAriu[a];Maria TeresaZedda[a];. Surgery on cervical folds for transcervical intrauterine artificial insemination with frozen-thawed semen enhances pregnancy rates in the sheep[J]. Theriogenology, 2019,126: 28-35