摘要
The increasing threat of antibiotic resistance in pathogenic bacteria and the dwindling supply of antibiotics available to combat these infections poses a significant threat to human health throughout the world. Antimicrobial peptides (AMPs) have long been touted as the next generation of antibiotics capable of filling the anti-infective void. Unfortunately, peptide-based antibiotics have yet to realize their potential as novel pharmaceuticals, in spite of the immense number of known AMP sequences and our improved understanding of their antibacterial mechanism of action. Recently, the immunomodulatory properties of certain AMPs have become appreciated. The ability of small synthetic peptides to protect against infection in vivo has demonstrated that modulation of the innate immune response is an effective strategy to further develop peptides as novel anti-infectives. This review focuses on the screening methods that have been used to assess novel peptide sequences for their antibacterial and immunomodulatory properties. It will also examine how we have progressed in our ability to identify and optimize peptides with desired biological characteristics and enhanced therapeutic potential. In addition, the current challenges to the development of peptides as anti-infectives are examined and the strategies being used to overcome these issues are discussed. © 2013 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 100: 572–583, 2013.
摘要译文
致病菌耐药性唱威胁，可用来对付这些感染的抗生素供应萎缩造成整个世界显著威胁人类健康。抗菌肽（安培）早已被吹捧为下一代能够填补抗感染无效的抗生素。不幸的是，基于肽的抗生素还没有意识到自己的潜力作为新型药品，尽管已知的AMP序列的巨大数量和它们的抗菌作用机制我们更好地理解的。近日，某些抗菌肽的免疫调节性能已成为赞赏。所有的合成肽，以防止感染在体内表明先天免疫应答的那个调制是一种有效的策略，以进一步开发的肽作为新型抗感染剂。这次审查的重点已用于评估它们的抗菌和免疫调节特性的新型肽序列筛选方法。它也将研究我们如何取得进展，我们来识别和优化肽所需的生物学特性和增强的治疗潜力的能力。此外，目前的挑战肽作为抗感染药物的开发进行检查和所使用的策略，以克服这些问题进行了讨论。生物聚合物（PEPT SCI）100：572-583，2013。
Evan F. Haney, Robert E. W. Hancock[*]. Peptide design for antimicrobial and immunomodulatory applications[J]. Peptide Science, 2013,100(6): 572-583