期刊文献

Effect of Alternative Splicing Euchromatic Histone Lysine Methyltransferase 2 (EHMT2/G9A) on Spermatogenesis in Mongolian Horses 收藏

替代剪接正念组蛋白赖氨酸甲基转移酶2（EHMT2/G9A）对蒙古马的精子发生的影响
原文求助 发布源
作者
Tergel Baatar [1];Dailing Song [2];Yajuan Weng [3];Guoqing Wang [4];Liangyi Jin [5];Rui Guo [6];Bei Li [7];Manglai Dugarjaviin [8];
作者单位
[1]Key Laboratory of Equus Germplasm Innovation (Coconstruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Hohhot 010018, China; [2]Key Laboratory of Equus Germplasm Innovation (Coconstruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Hohhot 010018, China; [3]Key Laboratory of Equus Germplasm Innovation (Coconstruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Hohhot 010018, China; [4]Key Laboratory of Equus Germplasm Innovation (Coconstruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Hohhot 010018, China; [5]Key Laboratory of Equus Germplasm Innovation (Coconstruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Hohhot 010018, China; [6]Key Laboratory of Equus Germplasm Innovation (Coconstruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Hohhot 010018, China; [7]Key Laboratory of Equus Germplasm Innovation (Coconstruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Hohhot 010018, China; [8]Key Laboratory of Equus Germplasm Innovation (Coconstruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Hohhot 010018, China;
发布日期
2025-4-11
页码
1106
DOI
10.3390/ani15081106
来源信息
Animals ISSN：2076-2615, 2025年, 15卷, 8期, 1106页
摘要
The epigenetic regulation of gene expression through the covalent modification of histones is crucial for developing germline cells. To study the regulatory role of alternative splicing (AS) of euchromatic histone lysine methyltransferase 2 (EHMT2/G9A) in spermatogenesis in Mongolian horses, this study first examines the localization of the EHMT2 gene in testicular support cells and then predicts the higher-order structures of sequences with and without AS. Two types of lentiviral vectors for overexpression were subsequently constructed for the EHMT2 gene, one with AS and one without, to infect support cells. The proliferation and activity of infected cells were measured using CCK8, and the differential expression of spermatogenesis-related genes in the two types of support cells was analyzed via qRT–PCR. We analyzed the expression of EHMT2 by immunofluorescence staining. EHMT2 was expressed in the nuclei of Sertoli cells. The expression of spermatogenesis-related genes was measured in the two types of cells. The results reveal that the expression levels of the FSH, Stra8, CCNB2, CDC27, NRG1, PPP2R5C, CCNB2, and YWHAZ genes in the AS group were greater than those in the control group. These results indicate that AS events in EHMT2 affect gene expression and thus affect spermatogenesis.
摘要译文
通过组蛋白的共价修饰对基因表达的表观遗传调节对于发育中的生殖细胞至关重要。为了研究正式组蛋白赖氨酸甲基转移酶2（EHMT2/G9A）在蒙古马中的精子发生中的替代剪接（AS）的调节作用（AS），这项研究首先检查了睾丸支持细胞中EHMT2基因的定位，然后对较高的序列结构进行了较高的序列结构。随后为EHMT2基因构建了两种用于过表达的慢病毒载体，一种是As and and and and and and and，以感染支持细胞。使用CCK8测量感染细胞的增殖和活性，并通过QRT – PCR分析了两种类型的支持细胞中与精子发生相关基因的差异表达。我们通过免疫荧光染色分析了EHMT2的表达。EHMT2在Sertoli细胞的核中表达。在两种类型的细胞中测量了与精子发生相关基因的表达。结果表明，AS组中FSH，Stra8，CCNB2，CCNB2，CCNB2，CCNB2，CCNB2，NRG1，PPP2R5C，CCNB2和YWHAZ基因的表达水平大于对照组中的基因。这些结果表明，随着EHMT2中的事件会影响基因表达，从而影响精子发生。
Tergel Baatar [1];Dailing Song [2];Yajuan Weng [3];Guoqing Wang [4];Liangyi Jin [5];Rui Guo [6];Bei Li [7];Manglai Dugarjaviin [8];. Effect of Alternative Splicing Euchromatic Histone Lysine Methyltransferase 2 (EHMT2/G9A) on Spermatogenesis in Mongolian Horses[J]. Animals, 2025,15(8): 1106