摘要
Feed supplementation can represent a relevant strategy to raise reproductive indexes in water buffalo herds. When diets rich in lipids are offered to ruminants, there is a benefit to reproduction. Considering that palm kernel cake (PKC) and coconut meal (CM) are lipid-rich industrial by-products, this study aimed to evaluate the effect of concentrates made of PKC or CM on the nutrient intake by water buffalos (Bubalus bubalis), to investigate their effects on semen characteristics, and to correlate semen parameters to the supplementary intake of lipids, crude protein, and minerals. Fifteen water buffalos kept on pasture were used as semen donors. The animals received daily dietary supplementation (1% of body weight) for 252 days, and were divided into three groups: Control (n = 5; conventional concentrate), CM-Base (n = 5; coconut meal concentrate), and PKC-Base (n = 5; palm kernel cake concentrate). Aspect, color, volume, pH, gross motility, vigor, progressive sperm motility, plasma membrane integrity, and sperm morphology of ejaculates were evaluated (173 samples). The daily average for voluntary concentrate intake was 4.778 ± 1.233 kg in the Control group, 3.112 ± 0.693 kg in CM-Base, and 4.558 ± 1.077 kg in PKC-Base group (P > 0.05). Diet based on palm kernel cake supplementation had a positive effect on semen quality. The plasma membrane integrity levels were increased in PKC-Base animals (Control: 68.0 ± 19.5; CM-Base: 72.0 ± 22.6; PKC-Base: 82.1 ± 12.2; P < 0.05). In addition, progressive sperm motility was higher in the PKC-Base group (71.7 ± 15.1% PKC-Base; 59.3 ± 20.5% Control; 56.7 ± 24.8% CMBase; P < 0.05). Lower sperm concentration values were observed in the PKC-Base group, but they did not deviate from the physiological standards from 524.10 ± 20.70 to 1,031.4 ± 28.70 x 106 sptz/ml, or of 1,493 x 106 sptz/ml. The other sperm parameters did not have significant differences (P > 0.05). Hence, palm kernel cake-based dietary supplementation for water buffalos provided more lipids and minerals, which intake is related to the improvement of sperm quality, with higher sperm motility and higher levels of spermatozoa with plasma membrane integrity
摘要译文
饲料补充可以代表一种相关的策略，以提高水牛群中的生殖指标。当向反刍动物提供富含脂质的饮食时，繁殖繁殖。考虑到棕榈仁蛋糕（PKC）和椰子粉（CM）是富含脂质的工业副产品，这项研究旨在评估PKC或CM对水牛（Bumalus Bubalis）的营养摄入量的浓缩物的效果。研究它们对精液特征的影响，并将精液参数与脂质，粗蛋白和矿物质的补充摄入相关。在牧场上保存的十五个水牛被用作精液供体。这些动物每天膳食补充剂（体重1％）252天，分为三组：对照（n = 5;常规浓缩物），CM碱（n = 5;椰子膳食浓缩物）和PKC碱（n = 5;棕榈仁蛋糕浓缩物）。评估了各方面，颜色，体积，pH，毛鳞状运动，活力，渐进精子运动，血浆膜完整性和射精的精子形态（173个样品）。对照组自愿浓缩物摄入的每日平均值为4.778±1.233千克，CM碱基3.112±0.693千克，PKC碱基组4.558±1.077千克（P> 0.05）。基于棕榈仁蛋糕补充的饮食对精液质量产生了积极影响。 PKC-基础动物中血浆膜完整性水平增加（对照：68.0±19.5; CM-BASE：72.0±22.6; PKC碱：82.1±12.2; P <0.05）。此外，PKC碱基组中进行的渐进精子活性较高（PKC碱71.7±15.1％; 59.3±20.5％的控制; 56.7±24.8％cmbase; P <0.05）。在PKC碱基组中观察到较低的精子浓度值，但它们没有偏离524.10±20.70至1,031.4±28.70 x 106 sptz / ml，或1,493 x 106 sptz / ml的生理标准。其他精子参数没有显着差异（p> 0.05）。因此，水牛的棕榈仁蛋糕膳食补充剂提供了更多的脂质和矿物质，其中摄入与精子质量的提高有关，具有较高的精子运动和具有血浆膜完整性的更高水平的精子
A.X. Santos;P.R. Kahwage;C. Faturi;T. Quinzeiro Neto;J.B. Lourenço Junior;M.R.S.P. Joele;A.R. Garcia. Feed supplementation with palm kernel cake-based concentrate increases the quality of water buffalo semen[J]. Animal Reproduction, 2014,11(2): 85-95