期刊文献

Molecular (ticks) and serological (humans) study of Crimean-Congo hemorrhagic fever virus in the Iberian Peninsula, 2013–2015 收藏

2013-2015伊比利亚半岛克里米亚 - 刚果出血热病毒的分子（蜱）和血清学（人类）研究

原文求助发布源

作者

Ana M. Palomar[a]; Aránzazu Portillo[a]; Sonia Santibáñez[a]; Lara García-álvarez[a]; Agustín Muñoz-Sanz[b]; Francisco J. Márquez[c]; Lourdes Romero[a]; José M. Eiros[d]; José A. Oteo[a]

作者单位

[a] Centro de Rickettsiosis y Enfermedades Transmitidas por Artrópodos Vectores, Departamento de Enfermedades Infecciosas, Hospital San Pedro-CIBIR, Logroño, Spain [b] Servicio de Patología Infecciosa, Hospital Universitario de Badajoz, Badajoz, Spain [c] Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Facultad de Ciencias Experimentales, Jaén, Spain [d] Facultad de Medicina, Universidad de Valladolid, Microbiología, Valladolid, Spain Received 1 December 2016, Accepted 30 January 2017, Available online 11 March 2017

关键词

Crimean-Congo hemorrhagic fever (CCHF); Crimean-Congo hemorrhagic fever virus (CCHFV); CCHFV IgG; Ticks; Hyalomma spp.; Rhipicephalus spp.; Spain

关键词译文

克里米亚 - 刚果出血热（CCHF）;克里米亚 - 刚果出血热病毒（CCHFV）; CCHFV IgG;蜱; Hyalomma spp。; Rhipicephalus spp。;西班牙

发布日期

11 March 2017

页码

344-347

DOI

10.1016/j.eimc.2017.01.009

来源信息

Enfermedades Infecciosas y Microbiología Clínica ISSN：0213-005X, 2017年, 35卷, 6期, 344-347页

摘要

Introduction Crimean-Congo hemorrhagic fever (CCHF) is a viral disease, mainly transmitted through tick bite, of great importance in Public Health. In Spain, Crimean-Congo hemorrhagic fever virus (CCHFV) was detected for the first time in 2010 in Hyalomma lusitanicum ticks collected from deer in Cáceres. The aim of this study was to investigate the presence of CCHFV in ticks from Cáceres, and from other Spanish areas, and to evaluate the presence of antibodies against the virus in individuals exposed to tick bites. Methods A total of 2053 ticks (1333 Hyalomma marginatum, 680 H. lusitanicum and 40 Rhipicephalus bursa) were analyzed using molecular biology techniques (PCR) for CCHFV detection. The determination of specific IgG antibodies against CCHFV in 228 serum samples from humans with regular contact with ticks (at risk of acquiring the infection) was performed by indirect immunofluorescence assay. Results The CCHFV was not amplified in ticks, nor were antibodies against the virus found in the serum samples analyzed. Conclusion The absence of the CCHFV in the ticks studied and the lack of antibodies against the virus in individuals exposed to tick bites would seem to suggest a low risk of acquisition of human infection by CCHFV in Spain.

摘要译文

引言克里米亚 - 刚果出血热（CCHF）是一种病毒性疾病，主要通过蜱叮咬传播，在公共卫生中具有重要意义。在西班牙，克里米亚 - 刚果出血热病毒（CCHFV）于2010年首次在卡塞雷斯的鹿中采集的Hyalomma lusitanicum蜱中被检测到。这项研究的目的是调查来自卡塞雷斯和其他西班牙地区的蜱中CCHFV的存在，并评估暴露于蜱叮咬的个体中抗病毒抗体的存在。方法使用分子生物学技术（PCR）分析总共2053个蜱（1333个Hyalomma marginatum，680个H. lusitanicum和40个Rhipicephalus法氏囊）用于CCHFV检测。通过间接免疫荧光测定法，在经常接触蜱（有感染风险）的228份血清样品中测定针对CCHFV的特异性IgG抗体。结果CCHFV未在蜱中扩增，在分析的血清样品中也未发现针对病毒的抗体。结论在研究的蜱中缺乏CCHFV并且在暴露于蜱叮咬的个体中缺乏针对病毒的抗体似乎表明西班牙CCHFV获得人感染的风险较低。

Ana M. Palomar[a]; Aránzazu Portillo[a]; Sonia Santibáñez[a]; Lara García-álvarez[a]; Agustín Muñoz-Sanz[b]; Francisco J. Márquez[c]; Lourdes Romero[a]; José M. Eiros[d]; José A. Oteo[a]. Molecular (ticks) and serological (humans) study of Crimean-Congo hemorrhagic fever virus in the Iberian Peninsula, 2013–2015[J]. Enfermedades Infecciosas y Microbiología Clínica, 2017,35(6): 344-347