摘要
Different media formulations and serum concentrations were tested for effect on yield of polyhedrin or a recombinant β-galactosidase fusion proteins produced in Spodoptera frugiperda cells infected with wild-type Autographa californica nuclear polyhedrosis virus or a genetically engineered baculovirus expression vector, respectively. Infected cells grown in Grace's medium supplemented with 0.5% fetal bovine serum (FBS) produced the highest levels of each of the proteins tested. A significant increase in protein synthesis was also observed when using serum-free Grace's medium or TNMFH medium supplemented with 0.5% FBS. The defined media and reduced serum or serum-free conditions increase levels of protein expression, lower production costs, and improve the ease of protein purification procedures for researchers using the baculovirus expression vector and producers of viral insecticides.
摘要译文
测试不同的培养基配方和血清浓度对分别用野生型苜蓿银纹夜蛾核多角体病毒或基因工程杆状病毒表达载体感染的草地贪夜蛾（Spodoptera frugiperda）细胞中产生的多角体蛋白或重组β-半乳糖苷酶融合蛋白的产量的影响。在补充有0.5％胎牛血清（FBS）的Grace培养基中生长的感染细胞产生了所测试的每种蛋白质的最高水平。当使用补充有0.5％FBS的无血清Grace培养基或TNMFH培养基时，也观察到蛋白质合成的显着增加。定义的培养基和降低的血清或无血清条件增加了蛋白质表达水平，降低了生产成本，并且改善了使用杆状病毒表达载体和病毒杀虫剂生产者的研究人员的蛋白质纯化程序的简易性。
Dana R.Broussard;Max D.Summers;. Effects of serum concentration and media composition on the level of polyhedrin and foreign gene expression by baculovirus vectors[J]. Journal of Invertebrate Pathology, 1989,54(2): 144-150