摘要
Insect cells (Spodoptera frugiperda) have been cultured in a stationary bed reactor, packed with a fibrous polyester carrier. When the bioreactor was perfused with serum-supplemented medium, a cell density of 6 × 106 cells ml−1 packed carrier was reached. Scanning electron microscopy investigations have shown that the insect cells grew along the three-dimensionally oriented fibers of the Fibra-cel carrier. After infection of the logarithmically growing cells with a recombinant baculovirus (Autographa californica) containing the gene coding for β-galactosidase, the medium in the bioreactor was changed to serum-free medium. At day 13 postinfection (p.i.), a β-glactosidase level of 320 μg ml−1 and, at day 17 p.i., a virus titer of 2.1 × 108 TCID50 units ml−1 (day 17 p.i.) were reached. In another bioreactor, operated in a similar way but with serum-containing medium, a β-galactosidase concentration of 360 μg ml−1 and a virus titer of 2.3 × 108 TCID50 units ml−1 were obtained. These results indicate the potential use of this production system for the production of recombinant protein and baculovirus in insect cells.
摘要译文
昆虫细胞（草地夜蛾（Spodoptera frugiperda））已在固定床反应器中培养，该反应器填充有纤维状聚酯载体。当生物反应器用补充血清的培养基灌注时，达到细胞密度为6×10 6 细胞ml -1 包装载体。扫描电子显微镜研究表明，昆虫细胞沿着Fibra-cel载体的三维取向纤维生长。用含有编码β-半乳糖苷酶的基因的重组杆状病毒（Autographa californica）感染对数生长的细胞后，将生物反应器中的培养基更换为无血清培养基。在感染后第13天（pi），β-半乳糖苷酶水平为320μg/ ml，在第17天，病毒滴度为2.1×10 8 TCID 50达到单位ml -1 （第17天）。在另一个生物反应器中，以类似方式操作但含有含血清的培养基，β-半乳糖苷酶浓度为360μg/ ml -1 ，病毒滴度为2.3×10 8 获得TCID 50单位ml -1 。这些结果表明该生产系统可用于在昆虫细胞中生产重组蛋白和杆状病毒。
R.Kompier[∗];N.Kislev[†];I.Segal[†];A.Kadouri[∗];. Use of a stationary bed reactor and serum-free medium for the production of recombinant proteins in insect cells[J]. Enzyme and Microbial Technology, 1991,13(10): 822-827