摘要
The phytochemical profile and antioxidant activities of Prunus africana bark, Leea indica and Paullinia pinnata leaves from Cameroon were investigated in this study. The yields of pure methanolic extraction were 11.9%, 11.1% and 10.8% in P. africana bark, L. indica and P. pinnata leaves, respectively. The total phenolic content was 189.0 ± 16.93, 163.6 ± 14.73 and 114.6 ± 10.38 mg GAE/g and total flavonoid content was 43.25 ± 6.43, 28.31 ± 4.44, and 19.75 ± 4.03 mg RU/g in P. africana bark, L. indica and P. pinnata leaves, respectively. The antioxidant activities of the plants were evaluated by DPPH, ABTS and FRAP assays. The IC50 evaluated in P. africana bark, L. indica and P. pinnata leaves was 109.5 ± 13.2, 132.1 ± 18.7 and 156.1 ± 21.9 µg/mL for DPPH and 98.1 ± 4.8, 101.3 ± 12.1 and 133.9 ± 16.0 µg /mL for ABTS assay. The FRAP value was 61.1 ± 1.5, 50.5 ± 1.5 and 43.4 ± 2.1 µMFe2+/g in the same sequence. The functional groups for the corresponding phytochemicals, including alkane, alkene, aliphatic ether, ester, amine, α, β-unsaturated ester, alcohol, phenol, carboxylic acid, and aliphatic ketone, were identified through fourier-transform infrared analysis. The identified and quantified phenolic acids in this study were methyl-4-hydroxybenzoic, caffeic, protocatechuic and p-coumaric acid, identified using high-performance liquid chromatography.
摘要译文
在这项研究中，研究了喀麦隆的Prunus Africana Bark，Leea Indica和Paullinia pinnata叶的植物化学谱和抗氧化活性。纯甲醇提取的产量分别为Africana Bark，L。Indica和P. pinnata叶片的11.9％，11.1％和10.8％。酚类总含量为189.0±16.93，163.6±14.73和114.6±10.38 mg gae/g/g，总类黄酮含量为43.25±6.43、28.31±4.44，19.75±4.03 mg ru/g in Africana bark in Africana bark in Africana Bark，L。Indica and P. pinnata asse commenity p. pinnata shebanta shem shemanta。通过DPPH，ABT和FRAP测定法评估了植物的抗氧化活性。DPPH的Africana Bark，L。Indifa和P. pinnata叶子在DPPH中进行评估的IC50为109.5±13.2、132.1±18.7和156.1±21.9 µg /ml，98.1±4.8，101.3，101.3±12.1和133.9±16.0 µg /ml for Abts Assay。在同一序列中，FRAP值为61.1±1.5、50.5±1.5和43.4±2.1 µmfe2+/g。通过傅立叶传递基础形成分析，鉴定了相应的植物化学物质的功能组，包括烷烃，烷烃，脂肪醚，酯，酯，胺，α，β-未饱和酯，β-β-未饱和酯，酒精，苯酚，羧酸和脂肪族酮。在这项研究中，已鉴定出的酚酸是使用高性能液相色谱鉴定的甲基-4-羟基苯甲酸，咖啡，原始技术和P-墨酸。