摘要
Injection of N-methyl-d-aspartate (NMDA, 7.5 μg) or quisqualate (2 μg) into the rat dorsal hippocampus induced wet-dog shakes and convulsions. As shown by an in situ immunohistochemical analysis, 3 h after the excitatory amino acids injections the rats displayed a bilateral profound elevation of the proenkephalin and prodynorphin mRNA levels in dentate gyrus granule cells (2–3 or 1.5–2 fold higher than control levels, respectively). Pretreatment of rats with d-amino-phosphonovalerate (d-APV, 10 μg), a selective antagonist of NMDA receptor, prevented the behavioral and biochemical changes evoked by NMDA. The changes in the behavior and gene expression evoked by kainate or quisqualate were diminished in rats which received 6-cyano-7-nitroquinoxaline-2,3-dion (CNQX, 2 μg), a putative antagonist of quisqualate and kainate receptors. The study demonstrated that activation of NMDA, quisqualate or kainate receptors in the hippocampus induced seizures associated with a marked increase in the proenkephalin (PENK) and the prodynorphin (PDYN) gene expression in the rat dentate gyrus.
摘要译文
向大鼠背侧海马注射N-甲基-d-天冬氨酸（NMDA，7.5μg）或quisqualate（2μg）诱导湿狗晃动和抽搐。如原位免疫组织化学分析所示，在兴奋性氨基酸注射后3小时，大鼠在齿状回颗粒细胞中显示出脑啡肽原和前体啡肽mRNA水平的双侧深度升高（比对照水平高2-3或1.5-2倍，分别）。用d-氨基 - 膦酰戊酸（d-APV，10μg）（一种NMDA受体的选择性拮抗剂）预处理大鼠，可以防止NMDA引起的行为和生化变化。在接受6-氰基-7-硝基喹喔啉-2,3-二酮（CNQX，2μg）（一种推定的quisqualate和红藻氨酸受体拮抗剂）的大鼠中，红藻氨酸盐或quisqualate引起的行为和基因表达的变化减弱。该研究表明，海马中NMDA，quisqualate或红藻氨酸受体的激活诱导的癫痫发作与大鼠齿状回中的脑啡肽原（PENK）和前体啡肽（PDYN）基因表达显着增加有关。
W.Lasoń;B.Przewlocka;R.Przewlocki;. The effects of excitatory amino acids on proenkephalin and prodynorphin mRNA levels in the hippocampal dentate gyrus of the rat; an in situ hybridization study[J]. Molecular Brain Research, 1992,12(1-3): 243-247