期刊文献

Tissue-Specific Expression of the Porcine DHRS3 Gene and Its Impact on the Proliferation and Differentiation of Myogenic Cells 收藏

猪DHRS3基因的组织特异性表达及其对肌原细胞增殖和分化的影响

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作者

作者单位

[1]Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; [2]Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; [3]Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; [4]Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; [5]Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; [6]Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; [7]Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; [8]Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; [9]Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China;

发布日期

2025-4-10

页码

1101

DOI

10.3390/ani15081101

来源信息

Animals ISSN：2076-2615, 2025年, 15卷, 8期, 1101页

摘要

The DHRS3 gene, a member of the short-chain dehydrogenase/reductase (SDR) family, is involved in critical metabolic processes in animals. This study investigated the expression patterns of DHRS3 across various tissues of developmental stages in pigs and preliminarily evaluated its effects on myoblast proliferation, apoptosis, and differentiation. RT-qPCR (real-time quantitative PCR) was employed to analyze DHRS3 expression in the heart, liver, spleen, lungs, kidneys, longissimus dorsi, foreleg, and hind leg of pigs at 3 days, 6 months, and 12 months of age. Cell proliferation was analyzed using EdU (5-ethynyl-2′-deoxyuridine) assays, RT-qPCR, and flow cytometry, while the expression changes of proliferation-, apoptosis-, and differentiation-related genes were assessed via RT-qPCR. The results indicated that DHRS3 was expressed in all eight tissues at all three developmental stages. At 3 days, DHRS3 expression was the highest in the kidneys; at 6 months, it peaked in the liver; and at 12 months, it was again the highest in the kidneys. Across all stages, the liver and kidneys exhibited the highest DHRS3 expression levels. Functional studies revealed that DHRS3 overexpression suppressed myoblast proliferation and differentiation while promoting apoptosis. In contrast, DHRS3 inhibition enhanced myoblast proliferation and differentiation and reduced apoptosis. These findings underscore the regulatory role of DHRS3 in myogenesis and provide insights into its metabolic and developmental functions in pigs.

摘要译文

DHRS3基因是短链脱氢酶/还原酶（SDR）家族的成员，参与动物的关键代谢过程。这项研究研究了猪发育阶段各个组织中DHRS3的表达模式，并初步评估了其对肌细胞增殖，凋亡和分化的影响。RT-QPCR（实时定量PCR）用于分析心脏，肝脏，脾脏，肺，肾脏，肾脏，龙龙，前龙，前法和后腿在3天，6个月和12个月大的猪中的DHRS3表达。使用EDU（5-乙基-2'-脱氧尿苷），RT-QPCR和流式细胞仪分析细胞增殖，而通过RT-QPCR评估了分化 - 凋亡 - 凋亡 - 凋亡和分化相关基因的增殖变化。结果表明，在所有三个发育阶段，在所有八个组织中均表达DHRS3。在3天时，DHRS3表达在肾脏中最高。在6个月时，它在肝脏中达到峰值。在12个月时，它再次是肾脏中最高的。在所有阶段，肝脏和肾脏表现出最高的DHRS3表达水平。功能研究表明，DHRS3的过表达抑制了肌细胞增殖和分化，同时促进凋亡。相反，DHRS3抑制作用增强了成肌细胞增殖和分化以及凋亡减少。这些发现强调了DHRS3在肌发生中的调节作用，并提供了有关其代谢和发育功能在猪中的见解。

Jifeng Li [1];Yong Ruan (https://orcid.org/0000-0003-0895-1356) [2];Chuanmei Jiang [3];Jinkui Sun [4];Dongwei An [5];Bo Zhou [6];Huan Liu [7];Ziyang Li [8];Houqiang Xu (https://orcid.org/0000-0002-4696-5671) [9];. Tissue-Specific Expression of the Porcine DHRS3 Gene and Its Impact on the Proliferation and Differentiation of Myogenic Cells[J]. Animals, 2025,15(8): 1101