摘要
This study was conducted to identify marbling-related candidate genes in M. longissimus dorsi of high- and low-marbled Hanwoo. The longissimus dorsi muscles were selected for gene expression from eight Hanwoo steer carcasses based on crude fat content. In the analysis of variance, gene expression of five candidate genes, FABP4, SCD, , Titin and Nebulin was determined to be significantly different between high- and low-marbled Hanwoo steers (P < 0.0001). The Pik-4 and CaMK II genes were also shown to have a significant effect on crude fat content (P < 0.01). In the analysis of the differential expression between high- and low marbled groups, FABP4 gene expression was approximately 2 times higher in the high marbled group relative to the low marbled group. However, the and SCD gene were highly expressed in the low marbled group. In addition, Titin and Nebulin were highly expressed in the low marbled group when placed under relatively high shear force. Finally, the Pik-4 and CaM K II gene also displayed a high expression pattern in the low marbled group.
摘要译文
本研究旨在鉴定高大和低大理石Hanwoo的背阔肌（M. longissimus dorsi）中与大理石花纹相关的候选基因。基于粗脂肪含量，从八只Hanwoo阉牛胴体中选择背最长肌以进行基因表达。在方差分析中，确定了5个候选基因FABP4，SCD，Titin和Nebulin的基因表达在高大理石和低大理石的Hanwoo阉牛之间有显着差异（P <0.0001）。还显示Pik-4和CaMK II基因对粗脂肪含量具有显着影响（P <0.01）。在分析高大理石组和低大理石组之间的差异表达时，相对于低大理石组，高大理石组中FABP4基因表达大约高2倍。然而，SCD和SCD基因在低大理石纹组中高表达。此外，当在相对高的剪切力下放置时，Titin和Nebulin在低大理石纹组中高度表达。最后，Pik-4和CaM K II基因在低大理石纹组中也显示出高表达模式。
Lee, Seung-Hwan; Cho, Yong-Min; Lee, Sang-Hong; Kim, Bum-Soo; Kim, Nam-Kuk; Choy, Yeon-Ho; Kim, Kyoung-Hoon; Yoon, Du-Hak; Im, Seok-Ki; Oh, Sung-Jong; Park, Eung-Woo;. Identification of marbling-related candidate genes in M. longissimus dorsi of high- and low marbled Hanwoo (Korean Native Cattle) steers[J]. BMB Reports, 2008,41(12): 846-851