摘要
The objective of this study was to determine the effects of dietary supplementation with chitooligosaccharide (COS) on growth performance, nutrient digestibility, blood characteristics and immune response in lipopolysaccharide-challenged weanling pigs. A total of 90 crossbred weanling pigs (5.44 ± 0.50 kg BW) were employed in Exp. 1. The three dietary treatments were basal diets supplemented with 0, 2.5, and 5 g COS/kg, and fed for 28 d. Each treatment had 6 replications with 5 pigs per pen. Increasing the level of supplemental COS tended to linearly (P 0.10) improve ADG and ADFI during phase 2 and overall period, while there were no differences in G:F. The linear improvement in the apparent DM (P 0.05) and N (P 0.10) digestibility in pigs fed COS supplemented diets was noticed. The tested blood characteristics were not influenced under non-challenge conditions. In Exp. 2, a total of 20 pigs (5.22 ± 0.31 kg BW) were initially assigned to two dietary treatments and fed basal diets supplemented with 0 or 0.5 g COS/kg for 28 d. At the end of d 28, half of the pigs in each treatment (n = 5) were injected i.p. with Escherichia coli lipopolysaccharide at a concentration of 100 μg/kg of BW. The other half of the pigs in each treatment were injected with sterile saline solution at a concentration of 100 μg/kg of BW. This arrangement resulted in a 2 × 2 factorial design with diet and LPS challenge as the main effects. Blood sample and rectal temperature data were collected at 0, 2, 4 and 12 h post-challenge. Rectal temperatures increased as the result of LPS injection at 4 and 12 h post-challenge (P 0.05). Serum cortisol, IGF-1, and TNF-α concentration were also increased as the result of LPS challenge (P 0.05). The COS treatments resulted in lower cortisol concentrations at 2 h and higher IGF-1 concentrations at 4 h post-challenge (P 0.05). COS and LPS interactions were also observed on cortisol and IGF-1 when the COS effects were presented (P 0.05). Haptoglobin concentrations remained unaffected throughout the challenge period. White blood cell counts were increased in the LPS-treated pigs at 2 and 4 h post-challenge (P 0.01). Lymphocyte count was elevated at 2 h and reduced at 12 h post-challenge as the result of LPS challenge (P 0.05). However, there were no COS main effects observed on lymphocyte count throughout the challenge period. The comparison between two LPS challenged treatments also indicated that COS treatment has beneficial effects on rectal temperature, cortisol and IGF-1 concentrations. In conclusion, dietary supplementation with COS had little effect on nutrient digestibility and inflammatory stress markers in weanling pigs.
摘要译文
本研究的目的是确定日粮添加壳寡糖（COS）对脂多糖激发断奶仔猪生长性能，营养物质消化率，血液特性和免疫反应的影响。在Exp中共使用了90头杂交断奶仔猪（5.44±0.50 kg BW）。 1.三种日粮处理均为基础日粮，添加0,2.5和5 g COS / kg，饲喂28 d。每次处理重复6次，每只猪5只。增加补充COS的水平趋向于线性（P = 0.10）在第2阶段和整个期间改善ADG和ADFI，而G：F没有差异。注意到饲喂COS补充日粮的猪的表观DM（P <0.05）和N（P 0.10）消化率的线性改善。在非攻击条件下，测试的血液特征不受影响。在Exp。 2，总共20头猪（5.22±0.31千克体重）最初分配到两个饮食处理，并喂养补充0或0.5克COS / kg的基础日粮28天。在第28天结束时，每次处理中一半的猪（n = 5）腹膜内注射。用浓度为100μg/ kg BW的大肠杆菌脂多糖。每个处理中另一半的猪注射浓度为100μg/ kg BW的无菌盐水溶液。这种安排导致2×2因子设计，以饮食和LPS挑战为主要影响。在攻击后0,2,4和12小时收集血液样品和直肠温度数据。在攻击后4和12小时，LPS注射导致直肠温度升高（P <0.05）。 LPS攻击后血清皮质醇，IGF-1和TNF-α浓度也增加（P <0.05）。 COS处理在攻击后4小时导致2小时皮质醇浓度降低和IGF-1浓度升高（P <0.05）。当提出COS效应时，在皮质醇和IGF-1上也观察到COS和LPS相互作用（P <0.05）。在整个挑战期间，触珠蛋白浓度保持不受影响。在攻击后2和4小时，LPS处理的猪的白细胞计数增加（P <0.01）。作为LPS攻击的结果，淋巴细胞计数在攻击后2小时升高并且在攻击后12小时降低（P <0.05）。然而，在整个攻击期间没有观察到对淋巴细胞计数的COS主效应。两种LPS攻击治疗之间的比较也表明COS治疗对直肠温度，皮质醇和IGF-1浓度具有有益作用。总之，膳食补充COS对断奶仔猪的营养消化率和炎症应激指标几乎没有影响。
Y.J.Chen;I.H.Kim;J.H.Cho;J.S.Yoo;Y.Wang;Y.Huang;H.J.Kim;S.O.Shin;. Effects of chitooligosaccharide supplementation on growth performance, nutrient digestibility, blood characteristics and immune responses after lipopolysaccharide challenge in weanling pigs[J]. Livestock Science, 2009,124(1-3): 255-260