期刊文献

Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research 收藏

循环介导的等温扩增（LAMP）是禽病毒研究中有前途的护理诊断策略
原文求助 发布源
作者
Faiz Padzil;1;2 Abdul Razak Mariatulqabtiah;1;3;* Wen Siang Tan;1;4 Kok Lian Ho;5 Nurulfiza Mat Isa;1;3 Han Yih Lau;6 Jalila Abu;7;Kuo-Pin Chuang8;9;10;11;12Faiz Padzil1Laboratory of Vaccine;Biomolecules; Institute of Bioscience; Universiti Putra Malaysia; Serdang 43400; Selangor; Malaysia; moc.liamg@lizdapziaf (F.P.); ym.ude.mpu@natsw (W.S.T.); ym.ude.mpu@aziflurun (N.M.I.)2Institute for Medical Research; National Institute of Health; Setia Alam; Shah Alam 40170; Selangor; MalaysiaFind articles by Faiz Padzil
发布日期
2022 Jan
DOI
10.3390/ani12010076
来源信息
Animals : an Open Access Journal from MDPI ISSN：e0030-6835, 2022年, 12卷, 1期
摘要
Simple Summary Many of the existing screening methods of avian viruses depend on clinical symptoms and pathological gross examinations that still necessitate confirmatory microscopic testing. Confirmation of a virus is often conducted at centralized laboratories that are well-equipped with instruments for virus isolation, hemagglutinin inhibition, virus neutralization, ELISA, PCR and qPCR. These assays are known for their great accuracy and sensitivity, and hence are set as standard practices. Nevertheless, limitations arise due to the time, cost and on-site applicability. As the technology progresses, molecular diagnostics should be more accessible to isolated areas and even practicable for use by non-skilled personnel such as farmers and private breeders. One of the point-of-care diagnostic strategies to consider for such matters is loop-mediated isothermal amplification (LAMP).
摘要译文
简单的摘要禽病毒现有的许多现有筛查方法取决于临床症状和病理严重检查，这些检查仍然需要确认性微观测试。病毒的确认通常是在装备有病毒分离仪器，血凝素抑制，病毒中和，ELISA，PCR和QPCR的仪器的集中实验室进行的。这些分析以其巨大的准确性和敏感性而闻名，因此将其作为标准实践设定。然而，由于时间，成本和现场适用性，出现限制。随着技术的进行，分子诊断应该更容易适用于孤立区域，甚至可以切实可行，以供农民和私人育种者等非技能人员使用。考虑到此类问题的即时诊断策略之一是循环介导的等温扩增（LAMP）。
Faiz Padzil;1;2 Abdul Razak Mariatulqabtiah;1;3;* Wen Siang Tan;1;4 Kok Lian Ho;5 Nurulfiza Mat Isa;1;3 Han Yih Lau;6 Jalila Abu;7;Kuo-Pin Chuang8;9;10;11;12Faiz Padzil1Laboratory of Vaccine;Biomolecules; Institute of Bioscience; Universiti Putra Malaysia; Serdang 43400; Selangor; Malaysia; moc.liamg@lizdapziaf (F.P.); ym.ude.mpu@natsw (W.S.T.); ym.ude.mpu@aziflurun (N.M.I.)2Institute for Medical Research; National Institute of Health; Setia Alam; Shah Alam 40170; Selangor; MalaysiaFind articles by Faiz Padzil. Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research[J]. Animals : an Open Access Journal from MDPI, 2022,12(1)