摘要
This study was aimed to search new genetic variants in the bovine FABP4 gene as molecular markers for meat quality and carcass traits. PCR–RFLP analysis revealed that three SNPs located at nucleotide positions g.2834C>G, g.3533T>A, and g.3691G>A were identified based on a GenBank accession number (NC_007312.4). Sequence analysis revealed that SNPs were located in intron 1 (g.2834C>G) and 2 (g.3533T>A), and an exon 3 (g.3691G>A), showing allele frequencies as 0.592, 0.579, and 0.789, respectively. Genetic variabilities of heterozygosity (He) and polymorphic information contents (PIC) were estimated for g.2834C>G (0.608 and 0.531), g.3533T>A (0.615 and 0.539), and g.3691G>A (0.498 and 0.401) loci, respectively. A SNP located in the exon 3 of FABP4 was characterized and associated with desirable increases of MS (marbling scores) and MG (meat quality grades) in Hanwoo. The statistical analysis revealed that additive effects by GG genotypes in g.3691G>A SNP were significantly greater than AA genotypes in MS and MG traits. These findings suggest that the FABP4g.3691G>A SNP will be a useful candidate locus to maximize economic benefits for cattle populations.
摘要译文
这项研究的目的是在牛FABP4基因分子标记的肉质和胴体性状寻找新的基因变异。PCR-RFLP分析显示，3个SNP位于核苷酸位置g.2834C序列分析表明，SNP位点均位于内含子1（g.2834C杂（他）和多态信息含量（PIC）的遗传变异进行了估算g.2834C498和0.401）位点，分别为。位于FABP4的外显子3一个SNP的特点，并与MS的需要增加（大理石纹评分）和MG（肉质等级）的HANWOO有关。统计分析表明，由GG基因型g.3691G累加效应3691G
Sung-Chul Shin [1] Jae-Pil Heo [1] Eui-Ryong Chung [1]. Genetic variants of the FABP4 gene are associated with marbling scores and meat quality grades in Hanwoo (Korean cattle)[J]. Molecular Biology Reports, 2012,39(5): 5323-5330