摘要
Three goats fitted with cannula were used to provide rumen liquor to investigate the effects of limiting amino acids on rumen fermentation and microbial community in vitro. The removal method was used in the current experiments. Treatments are total essential amino acid (TEAA), His-removal, Lys-removal, Met-removal, and branch chain amino acid (BCAA)- removal. Results indicated that, pH-value ranged between 5.9 and 6.8, with the highest mean value for the group with BCAA-removal (6.54) in the culture. Concentration of NH3-N ranged between 10.99 to 30.51 mg 100 mL−1, with the group of TEAA recording the highest average NH3-N concentration (17.85 mg 100 mL−1). Yields of microbial protein and limiting degree on microbial growth varied with treatments (P P
摘要译文
将三只装有套管的山羊用于提供瘤胃液，以研究限制氨基酸对瘤胃发酵和体外微生物群落的影响。在当前的实验中使用去除方法。治疗是总必需氨基酸（TEAA），去除His，去除Lys，去除Met和去除支链氨基酸（BCAA）。结果表明，pH值范围在5.9和6.8之间，培养物中BCAA去除组（6.54）的平均值最高。 NH 3 -N的浓度范围为10.99至30.51 mg 100 mL -1 ，TEAA组记录的NH 3 -N平均浓度最高（17.85 mg 100 mL -1 ）。微生物蛋白质的产量和对微生物生长的限制程度随处理而变化（P P
Meng-zhiWANG;Hong-rongWANG;Heng-chunCAO;Guo-xiangLI;JieZHANG;. Effects of Limiting Amino Acids on Rumen Fermentation and Microbial Community In vitro[J]. Agricultural Sciences in China, 2008,7(12): 1524-1531