摘要
The purpose of the study was to identify different viruses infecting sweetpotato and the level of co-infection and spatial distribution of the viruses within the Acholi sub-region of northern Uganda. Multiplex PCR was used to screen and determine level of co-infection in 380 sweetpotato plants. The PCR scores were computed to give overall frequency of occurrence of different viruses. The spatial distribution of viruses was represented on an ArcGIS map. Of all screened samples, 24% (92/380) were infected with at least one virus. Sweetpotato feathery mottle virus (65/92), sweetpotato chlorotic fleck virus (17/92) and sweetpotato mild mottle virus (8/92) were the most frequent viruses detected. Of sampled fields, 74% (28/38) had at least one virus-infected sweetpotato plant. The four viruses detected are the major viruses causing significant yield losses in major sweetpotato growing regions of Uganda and East Africa. The findings of limited distribution and low prevalence of the viruses in the region indicate it causes less burden to sweetpotato production in the sub-region compared with other parts of Uganda.
摘要译文
该研究的目的是确定感染甘薯的不同病毒以及乌干达北部Acholi亚区内病毒的共感染和空间分布水平。使用多重PCR筛选和确定380个甘薯植物中的共感染水平。计算PCR得分以给出不同病毒的总体发生频率。病毒的空间分布在ArcGIS地图上显示。在所有筛选的样品中，24％（92/380）被至少一种病毒感染。甘薯羽毛斑驳病毒（65/92），甘薯褪绿斑病毒（17/92）和甘薯轻微斑驳病毒（8/92）是最常检测到的病毒。在采样的田地中，74％（28/38）至少有一种受病毒感染的甘薯植物。检测到的四种病毒是导致乌干达和东非主要甘薯种植区域产量大幅下降的主要病毒。该地区病毒分布有限和流行率低的发现表明，与乌干达其他地区相比，该地区的甘薯生产负担较轻。
GodfreyWokorach[a][b][c];HilaryEdema[a];DennisMuhanguzi[b][1];RichardEchodu[c][1];. Prevalence of sweetpotato viruses in Acholi sub-region, northern Uganda[J]. Current Plant Biology, 2019,17: 42-47