摘要
The objective of this study was to evaluate the transferable embryo recovery rates from superovulated donor cattle after different artificial insemination (AI) schedules. Sixty mixed-breed crossbred females were administered follicle stimulating hormone (FSH) and prostaglandin F2α (PGF2α) to induce a superovulatory response. At standing estrus, donor females were randomly allotted to one of five treatment groups for AI. Donors were inseminated with two units of high-quality or low-quality frozen semen at 12, 24, 36, or 48 h after the onset of estrus in treatment Groups I, II, III, and IV, respectively, or inseminated with two units at 12, 24, 36, and 48 h (eight units/donor) in control Group V. Donor females inseminated once at either 12 or 24 h after the onset of estrus did not differ from donors inseminated in Group V in overall fertilization and transferable embryo recovery rates. The highest fertilization rate (89.5%) and transferable embryo recovery rate (74.9%) per donor resulted when AI was performed with high-quality semen at 24 h after the onset of estrus. These findings indicate that repeated insemination of superovulated beef cattle is not necessary to attain optimal fertilization rates and production of transferable quality embryos in beef cattle.
摘要译文
本研究的目的是评估不同人工授精（AI）计划后超排卵供体牛的可转移胚胎回收率。对60只混合品种的杂交雌性动物给予卵泡刺激素（FSH）和前列腺素F2α（PGF2α）以诱导超排反应。在发情期，将供体雌性随机分配到五个治疗组中的一个用于AI。在第I，II，III和IV组治疗发情后12,24,36或48小时，捐献者用两个高质量或低质量的冷冻精液进行授精，或者用两个单位进行授精在对照组V中12,24,36和48小时（8个单位/供体）。在发情后12或24小时给予雌性一次授精，与第V组在整个受精中受精的供体无差异并且可转移胚胎恢复率。在发情后24小时用高质量精液进行AI治疗后，每个供体的受精率最高（89.5％）和转移胚胎回收率（74.9％）。这些发现表明，超排卵肉牛的重复授精对于获得最佳的受精率和在肉牛中生产可转移的优质胚胎是不必要的。
M.C.Schiewe;C.R.Looney;C.A.Johnson;K.G.Hill;R.A.Godke;. Transferable embryo recovery rates following different insemination schedules in superovulated beef cattle[J]. Theriogenology, 1987,28(4): 395-406