摘要
The aim of this study was to identify the polymorphism on fatty acid binding protein (FABP4) gene promoter region and its association with carcass traits in Hanwoo. We performed PCR-direct sequencing of FABP4 promoter region to identify single nucleotide polymorphism (SNPs) using unrelated 24 Hanwoo bulls. Four SNPs (-298A>G, -472A>G, -887A>G, -862A>G) were detected in the promoter region and genotyped on 583 Hanwoo steers. A linear mixed model revealed an association of three SNPs (-298A>G, -472A>G and -862A>G) with carcass weight and marbling score in dominance model (P<0.05). The animals with AA genotypes for the three SNPs were heavier carcass weight (5 kg) than animals with GG genotypes in the statistical analysis. For the marbling score, the AA genotype was lower effect of marbling score (0.21) than GG genotypes. In conclusion, this study indicates an important role for three SNPs detected in promoter region of FABP4 in determining carcass weight and marbling score in Hanwoo.
摘要译文
本研究的目的是鉴定Hanwoo中脂肪酸结合蛋白（FABP4）基因启动子区域的多态性及其与胴体性状的关联。我们对FABP4启动子区域进行了PCR直接测序，以使用不相关的24个Hanwoo公牛鉴定单核苷酸多态性（SNP）。在启动子区域中检测到4个SNP（-298A> G，-472A> G，-887A> G，-862A> G）并在583个Hanwoo阉牛上进行基因分型。线性混合模型揭示了三个SNP（-298A> G，-472A> G和-862A> G）与优势模型中胴体重和大理石花纹评分的相关性（P <0.05）。在统计分析中，具有针对三个SNP的AA基因型的动物比具有GG基因型的动物具有更重的胴体重（5kg）。对于大理石花纹分数，AA基因型对大理石花纹分数（0.21）的影响低于GG基因型。总之，本研究表明在FOBP4的启动子区域中检测到的三个SNP在测定Hanwoo中胴体重和大理石花纹评分中的重要作用。
Lee, Seung-Hwan; Kim, Nam-Kuk; Kim, Seung-Chang; Choi, Bong-Hwan; Heo, Kang-Neung; Lee, Chang-Soo; Kim, Oun-Hyun; Lee, Jun-Heon; Kim, Hyeong-Cheul; Hong, Seong-Koo;. Genetic polymorphism in regulatory region of fatty acid binding protein 4 (FABP4) and its effect on carcass weight in Hanwoo steers[J]. Korean Journal of Agricultural Science, 2011,38(4): 673-680