摘要
Besides post-thawing reduced semen quality, there are some difficulties in the execution of the endoscopic transcervical intrauterine artificial insemination (AI) with frozen–thawed semen in bitches. Therefore, the aims of the present study were to evaluate behavioral and reproductive parameters (i.e., vaginal cytology and serum progesterone level) to determine time of insemination and to investigate the particularities and difficulties of this technique in bitches using fresh semen. Ten Labrador Retriever bitches were submitted to three endoscopic transcervical intrauterine AIs (with 48 h intervals). Oestrus and ovulation period were established by behaviour evaluation, progesterone assays and vaginal cytology, enabling optimal timing for AI during oestrus. During AI, the following aspects were evaluated: cervical os catheterization difficulty, semen deposition resistance, occurrence of semen backflow, and time required to perform the AI. In this study, it was possible to catheterize the cervical os in all bitches, with different degrees of difficulty, by manipulating the equipment to allow cervical visualization and catheter introduction in the cervical canal. Serial serum progesterone assays enabled estimation of LH surge day, and thus of ovulation. The pregnancy rate was 90%, with a litter size of 5.0 ± 2.6 puppies per bitch. It was concluded that the difficulties in the execution of the endoscopic transcervical intrauterine AI technique in Labrador Retriever bitches were minimized by the equipment manipulation and practical experience.
摘要译文
除了解冻后降低精液质量外，在母犬中使用冻融精液进行内窥镜子宫内人工授精（AI）也存在一些困难。因此，本研究的目的是评估行为和生殖参数（即阴道细胞学和血清孕酮水平）以确定授精时间并研究该技术在使用新鲜精液的母犬中的特殊性和困难。将十只拉布拉多猎犬母犬提交给三个内窥镜子宫内宫内AI（间隔48小时）。通过行为评估，孕酮测定和阴道细胞学确定发情和排卵期，从而在发情期间实现AI的最佳定时。在AI期间，评估了以下方面：宫颈导管插入困难，精液沉积抗性，精液回流的发生和执行AI所需的时间。在这项研究中，通过操纵设备以允许宫颈可视化和宫颈管中的导管引入，可以在所有母犬中插入具有不同难度的宫颈口。连续血清孕酮测定能够估计LH激增日，从而估计排卵。怀孕率为90％，每窝的窝产仔数为5.0±2.6只幼犬。结论是，通过设备操作和实践经验，最大限度地减少了在拉布拉多猎犬母犬中执行内窥镜子宫内宫内AI技术的困难。
Sabrina PereiraMacedo;ChristinaMalm;Marc Roger Jean MarieHenry;Luiz FlávioTelles;Mariana SilvaFigueiredo;Fabíola BonoFukushima;Mariana MachadoNeves;Guilherme Albuquerquede Oliveira Cavalcanti;Marcela SilvaChaves;Rebeca MarquesMascarenhas;Moniquede Albuquerque Lagares;Valentim ArabicanoGheller;. Endoscopic transcervical intrauterine artificial insemination in Labrador Retriever bitches[J]. Research in Veterinary Science, 2012,92(3): 494-500