摘要
【Abstract】 【Aims】 This study aimed to investigate the molecular characterization and antimicrobial susceptibility of Corynebacterium pseudotuberculosis from skin abscesses of Korean native black goats (KNBG, Capra hircus coreanae) in South Korea. 【Methods and results】 A total of 83 isolates were recovered from skin abscesses of KNBG. Of these isolates, 74 isolates were identified as C. pseudotuberculosis by phospholipase D (PLD) gene-based PCR assay. Each of the isolates possessed all 18 virulence genes (FagA, FagB, FagC, FagD, SigE, SpaC, SodC, PknG, NanH, OppA, OppB, OppC, OppD, OppF, CopC, NrdH and CpaE). The genetic diversity of C. pseudotuberculosis isolates was assessed by the phylogenetic analysis using the concatenated sequences (3073 bp) of five housekeeping genes (fusA, dnaK, infB, groeL1 and leuA) for investigating their genetic diversity. In the results, the isolates belonged to three groups: group 1 (67 isolates), group 2 (one isolate) and group 3 (six isolates) within biovar ovis. However, the groups exhibited low genetic diversity (0.20%–0.41%). In the antimicrobial susceptibility test, most isolates were susceptible to tetracycline, vancomycin, chloramphenicol, ciprofloxacin, erythromycin, enrofloxacin, cefoxitin, ampicillin, gentamycin, cephalothin and doxycycline, whereas they were not susceptible to cefotaxime, trimethoprim and streptomycin. 【Conclusion】 This results suggest the involvement of relatively few clones of C. pseudotuberculosis in Korea. Further, present isolates can threaten public health due to potentially virulent strains with all 18 virulence genes and non-susceptible strains to clinically important antibiotics (CIA) and highly important antibiotics. 【Significance and Impact of the Study】 This study is the first to investigate the genetic diversity and potential pathogenicity of C. pseudotuberculosis biovar ovis isolates from skin abscesses of KBNG in South Korea, and could provide useful information in controlling its infections.
摘要译文
【摘要】【旨在本研究旨在研究韩国韩国韩国韩国本地黑山羊（KNBG，capra hircus coreanae）皮肤脓肿的分子表征和抗菌敏感性。【方法和结果】从KNBG的皮肤脓肿中总共回收了83个分离株。在这些分离株中，通过基于磷脂酶D（PLD）基因的PCR测定法，将74个分离株鉴定为假结核梭菌。每个分离株都有所有18种毒力基因（FAGA，FAGB，FAGC，FAGD，SIGE，SIGE，SODC，SODC，PKNG，NANH，NANH，OPPA，OPPB，OPPB，OPPC，OPPD，OPPD，OPPF，COPC，COPC，NRDH，NRDH和CPAE）。通过使用系统发育分析评估了五个家政基因（FUSA，DNAK，INFB，GROEL1和LEUA）的偶发序列（3073 bp），通过系统发育分析（3073 bp）来评估遗传多样性。在结果中，分离株属于三组：第1组（67个分离株），第2组（一个分离株）和第3组（6个分离株（6个分离株））在生物娃娃ovis中。但是，这些组表现出低遗传多样性（0.20％–0.41％）。In the antimicrobial susceptibility test, most isolates were susceptible to tetracycline, vancomycin, chloramphenicol, ciprofloxacin, erythromycin, enrofloxacin, cefoxitin, ampicillin, gentamycin, cephalothin and doxycycline, whereas they were not susceptible to cefotaxime, trimethoprim and streptomycin.【结论】该结果表明，韩国较少的假性结核梭状芽胞杆菌的克隆涉及相对较少的克隆。此外，由于所有18种毒力基因和对临床上重要的抗生素（CIA）和非常重要的抗生素的潜在毒性菌株和不敏感的菌株，目前的分离株可能威胁到公共卫生。研究的意义和影响】这本研究是第一个研究北韩KBNG皮肤脓肿的假结核C. pseudotubolosis Biovar Ovis分离株的遗传多样性和潜在致病性的研究，并可以提供有用的信息来控制其感染。