摘要
Leaves of Pisum sativum L. cv. ‘Perfected Wales’ were inoculated with pea enation mosaic virus, at the 3rd and 4th nodes as early as size permitted. Systemic virus spread was then measured over a period of approximately 9 days by assaying the infectivity of 1-cm stem pieces above the 4th and below the 3rd node. The presence of virus antigen and double-stranded (ds) RNA in these stem pieces was measured by radioimmunoassay over the same period. Relative infectivity and amounts of dsRNA in infected tissue were lowest approximately 3 days after inoculation and sharply increased thereafter. A transient plasmodesmatal abnormality was observed 4–6 days after inoculation. The transient nature of the abnormality, which was preceded (24 h) by low infectivity and dsRNA concentrations, may indicate a plant defense reaction that is subsequently overcome. The rise in dsRNA concentration, starting the 4th day after inoculation, coincided with the increase of vesicular material (cytopathological structures) in the phloem of infected plants. Vesicular material was found in sieve elements that were mature at the time of inoculation. Their presence in this tissue can only be explained by transport of the vesicles from infected sites.
摘要译文
豌豆（Pisum sativum L.）的叶子。尽其所能，在第3和第4节点向“完美威尔士”接种了豌豆花叶病毒。然后，通过分析第4个节点以上和第3个节点以下的1 cm茎杆的感染性，在大约9天的时间内测量系统性病毒传播。同期通过放射免疫法检测这些茎片中病毒抗原和双链（ds）RNA的存在。接种后约3天，感染组织中的相对感染性和dsRNA的量最低，此后急剧增加。接种后4–6天观察到短暂的浆膜异常。低异常性和dsRNA浓度之前（24小时），异常的短暂性可能表明植物防御反应随后被克服。从接种后第4天开始，dsRNA浓度的升高与被感染植物韧皮部囊泡物质（细胞病理结构）的增加相吻合。在接种时成熟的筛子元件中发现了囊状物质。它们在该组织中的存在只能通过从感染部位转运囊泡来解释。
de Zoeten G.A. Gaard G.. Mechanisms Underlying Systemic Invasion of Pea Plants by Pea Enation Mosaic Virus[J]. Intervirology, 1983,19(2): 85-94