摘要
The objective of the current study was to evaluate the effects of rumen-protected lysine (RPL) supplementation on performance and plasma amino acids in Holstein cows. A total of 40, primi- and multiparous Holstein cows (DIM 49 ± 13) were assigned to 1 of 2 treatments in a randomized complete block design consisting of 20 blocks. Total length of the experiment was 49 d. From d1 to d7 cows were not fed RPL (adaptation phase; covariate) and from d8 to d49 (experimental phase) cows received the treatments that were top-dressed. Treatments were as follow: cows fed a basal TMR + 300 g of dried ground molasses (CON); and basal TMR + RPL (NPL; ADM, Decatur, IL) at 0.41% of the TMR DM. Blood samples were taken from the coccygeal vein or artery, on d 7, 14, 28, 42, 45, and 49 of each period from each cow. Statistical analysis was performed using the MIXED procedure in SAS (v9.4). There was no difference for DMI in cows in CON and NPL (P > 0.21). Cows in NPL had a tendency for lower BCS (3.22 ± 0.04) than CON (3.32 ± 0.03) (P = 0.06). There were no treatment differences for BW (P = 0.30) and milk protein [CON (2.94 % ± 0.04) and NPL (2.93 % ± 0.05) (P = 0.85)]. Cows in CON (64.35μM/L ± 2.06) had lower (P = 0.03) Lys plasma concentration then NPL (71.14 μM/L ± 2.11). Cows in CON (64.52 μM/L ± 1.35) had tendency for lower (P = 0.08) Arg concentration than NPL (67.98 μM/L ± 1.38). In conclusion, NPL did not improve nor decreased DMI, milk yield and components; but increased plasma Lys and Arg, concentrations in early lactation Holstein cows.
摘要译文
当前研究的目的是评估瘤胃保护赖氨酸（RPL）补充对荷斯坦母牛中性能和血浆氨基酸的影响。在由20个区块组成的随机完整块设计中，总共分配了40个原始荷斯坦奶牛（DIM 49±13），分配给2种处理中的1种。实验的总长度为49 d。从D1到D7母牛未喂食RPL（适应阶段；协变量），从D8到D49（实验阶段）奶牛接受了底漆的治疗方法。处理如下：奶牛喂养基底TMR + 300 g干地糖蜜（Con）；和基础TMR + RPL（NPL; ADM，Decatur，IL），占TMR DM的0.41％。在每个牛的D 7、14、28、42、45和49上，从尾静脉或动脉中取出血液样本。使用SAS中的混合程序进行统计分析（v9.4）。 Con和NPL中的母牛中的DMI没有差异（P> 0.21）。 NPL中的母牛比Con（3.32±0.03）（p = 0.06）的BC较低（3.22±0.04）的趋势。 BW没有治疗差异（P = 0.30）和牛奶蛋白[CON（2.94％±0.04）和NPL（2.93％±0.05）（P = 0.85）]。 CON（64.35μm/L±2.06）中的母牛的含量较低（P = 0.03）Lys等离子体浓度，而NPL（71.14μm/L±2.11）。 CON中的母牛（64.52μm/L±1.35）的趋势低于NPL（67.98μm/L±1.38）。总之，NPL并未改善或降低DMI，牛奶产量和成分。但是增加了血浆LYS和ARG，早期泌乳荷斯坦母牛的浓度。
Geoffrey Carney; Phil Cardoso; ; Perry Doane. PSVI-18 Effects of rumen-protected lysine on performance and plasma amino acid metabolism in early lactation Holstein cows[J]. Journal of Animal Science, 2020,98(Sup3): 216-217