摘要
Understanding the molecular mechanisms involved in plant virus–vector interactions is essential for the development of effective control measures for aphid-vectored epidemic plant diseases. The coat proteins (CP) are the main component of the viral capsids, and they are implicated in practically every stage of the viral infection cycle. Pea enation mosaic virus 1 (PEMV1, Enamovirus, Luteoviridae) and Pea enation mosaic virus 2 (PEMV2, Umbravirus, Tombusviridae) are two RNA viruses in an obligate symbiosis causing the pea enation mosaic disease. Sixteen mutant viruses were generated with mutations in different domains of the CP to evaluate the role of specific amino acids in viral replication, virion assembly, long-distance movement in Pisum sativum, and aphid transmission. Twelve mutant viruses were unable to assemble but were able to replicate in inoculated leaves, move long-distance, and express the CP in newly infected leaves. Four mutant viruses produced virions, but three were not transmissible by the pea aphid, Acyrthosiphon pisum. Three-dimensional modeling of the PEMV CP, combined with biological assays for virion assembly and aphid transmission, allowed for a model of the assembly of PEMV coat protein subunits.
摘要译文
了解植物病毒 - 载体相互作用中涉及的分子机制对于制定蚜虫传播的流行病植物疾病的有效控制措施至关重要。外壳蛋白（CP）是病毒衣壳的主要成分，它们几乎涉及病毒感染周期的每个阶段。豌豆花叶病毒1（PEMV1，Enamovirus，Luteoviridae）和Pea enation花叶病毒2（PEMV2，Umbravirus，Tombusviridae）是两种RNA病毒，是一种导致豌豆花叶病的专性共生病。产生了16种突变病毒，其在CP的不同结构域中具有突变，以评估特定氨基酸在病毒复制，病毒体装配，豌豆中的长距离运动和蚜虫传播中的作用。 12种突变病毒无法组装，但能够在接种的叶子中复制，移动长距离，并在新感染的叶子中表达CP。四种突变病毒产生病毒粒子，但三种不能通过豌豆蚜虫Acyrthosiphon pisum传播。 PEMV CP的三维建模，结合病毒体装配和蚜虫传播的生物学测定，允许PEMV外壳蛋白亚基组装的模型。
Juliette Doumayrou[ 1;†;][*] ; Melissa Sheber[ 1]; Bryony C. Bonning[ 2];W. Allen Miller[ 1]. Role of Pea Enation Mosaic Virus Coat Protein in the Host Plant and Aphid Vector[J]. Viruses, 2016,8(11): 312